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绝大多数含有核酸的脂质纳米颗粒含有货物

Rasmus Münter¹, Jannik B Larsen¹, Thomas L Andresen¹

¹Colloid and Biological Interfaces (CBIO), Department of Health Technology, Technical University of Denmark (DTU), Kgs. Lyngby, Denmark.

Journal of colloid and interface science

|June 26, 2024

概括

大多数脂质纳米粒子 (LNPs) 实际上都携带核酸载荷. 研究表明,不到16%的mRNA载荷的LNP是空的,这表明它们适合治疗.

科学领域:

纳米医学是一种纳米医学.
生物技术是生物技术.
分子疗法分子疗法

背景情况:

脂质纳米粒子 (LNP) 是基于核酸的疗法的关键输送载体.
纳米医学的一个重大问题是,很大一部分LNP可能是空的,缺乏治疗载荷.
没有充分了解LNP配方,制备方法,货物类型和空LNP的比例之间的关系.

研究的目的:

调查LNP中核酸负载的异质性.
为了确定不同核酸货物 (siRNA,mRNA,等离子体) 中空的LNP的比例.
评估LNP配方和制剂对空空LNP患病率的影响.

主要方法:

利用一种成熟的基于单颗粒的方法来分析LNP的组成.
在各种LNP配方中量化核酸负载效率.
检查的LNP载有小干扰RNA (siRNA),信使RNA (mRNA) 和等离子体.

主要成果:

发现LNP的一小部分是空的,不管核酸货物是什么.
对于临床相关的mRNA-LNP,空纳米颗粒的比例在分析组合中不超过16%.
标准的LNP脂质-货物组合和制备方法产生主要是装载的纳米粒子.

结论:

该研究表明,常用的LNP配方和制备技术导致有效的核酸封装.
空的LNP的流行率是最小的,这表明标准的LNP具有有效的生物医学应用的潜力.
这些发现减轻了对LNP货物加载效率用于治疗目的的担忧.

关键词:

毒品装载毒品装载脂质纳米颗粒是一种纳米粒子.纳米颗粒的特征表征纳米粒子异质性的不同.配送RNA配送RNA的时间单个粒子研究.

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