在Acetobacterium woodii中重定向电子流使CO的生长成为可能,并改善格式的生长
Jimyung Moon1, Anja Poehlein2, Rolf Daniel2
1Department of Molecular Microbiology & Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe University, Max-von-Laue Str. 9, Frankfurt, Germany.
Nature communications
|June 26, 2024
概括
经过基因改造而缺乏特定化酶的Acetobacterium woodii现在可以在一氧化碳上生长,一氧化碳是以前无法使用的原料. HDCR酶的突变有助于这种适应,增强生物质生产.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 合成生物学 合成生物学
背景情况:
- 无氧性,乙酸性细菌将单碳化合物转化为有价值的产品,如乙酸和生物燃料.
- 一个模型的乙烯基原体Acetobacterium woodii使用的是二氧化碳,甲酸盐或甲醇,而不是一氧化碳 (CO).
- 已知基酶被CO抑制,限制其作为原料的使用.
研究的目的:
- 通过基因修改其酶通路,使Acetobacterium woodii能够在一氧化碳 (CO) 上生长.
- 研究A. woodii在使用CO作为唯一碳来源时的适应机制.
- 探索化酶删除对二氧化碳代谢和生物质产量的影响.
主要方法:
- 在Acetobacterium woodii中的[FeFe]基酶HydA2和HydBA的遗传删除.
- 在CO上培养突变菌株作为唯一的碳来源.
- 单核酸多态性 (SNP) 分析以确定CO培养细胞中的适应性突变.
- 对依赖的二氧化碳还原酶 (HDCR) 活性进行分析.
主要成果:
- ∆hydBA/hydA2突变体在经过显著的适应期后,在CO上表现出增长,产生酸盐.
- 在SNP分析中,发现了CO适应细胞中HDCR的HycB2亚单元的突变.
- 在没有HydA2模块的情况下,HDCR显示了变化的活性,铁素依赖的CO2减少增加.
- 与野生类型相比,这种适应CO的突变物在成型物上生长时,其最终生物质产量翻了一番.
结论:
- 特定基酶的遗传删除和随后的适应使Acetobacterium woodii能够利用一氧化碳.
- 对于CO适应和改变CO2代谢而言,HDCR的HycB2子单元的突变至关重要.
- 这项研究扩大了A. woodii在可持续生物技术方面的潜力,通过使用工业废物CO.
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