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Updated: Jun 22, 2025

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
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通过增强Bxb1血清整合酶的核定位,提高了CHO细胞的稳定整合效率

Olli Huhtinen1,2, Stuart Prince3,4, Urpo Lamminmäki5

  • 1Protein & Antibody Engineering, Orion Corporation, Turku, Finland. olanhu@utu.fi.

BMC biotechnology
|June 26, 2024
PubMed
概括

用核定位信号 (NLS) 和RanGAP提高Bxb1整合酶的效率,显著提高了哺乳动物显示库的稳定基因组整合. 这种方法将整合效率提高了14倍以上,使得更大的抗体开发库成为可能.

关键词:
Bxb1 血清整合酶的作用哺乳动物展示展示核定位信号是一个核定位信号.

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