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三合体和独特的基因表达赋予了Vitis labrusca葡萄藤中的昆虫草食耐药性

Cullen W Dixon^1,2, Andrea R Gschwend³

¹Department of Horticulture and Crop Science, The Ohio State University, Columbus, OH, 43210, USA.

BMC plant biology

|June 26, 2024

概括

维蒂斯拉布鲁斯卡"GREM4"由于三体密度较高和独特的基因表达模式,包括防御和二次代谢物生物合成,表现出优越的日本甲虫耐药性. 这为开发更有弹性的葡萄树提供了洞察力.

科学领域:

植物科学植物科学
遗传学遗传学是一种遗传学.
昆虫学昆虫学是一门学科.

背景情况:

葡萄藤 (Vitis) 是一种重要的水果作物,受到昆虫食草的威胁,需要加强耐药性的策略.
维蒂斯·拉布鲁斯卡 (Vitis labrusca) 是一个野生的北美物种,是开发具有改善抗压力葡萄树的宝贵遗传资源,以实现可持续的葡萄种植.

研究的目的:

为了评估Vitis labrusca acc. 的耐药性. "GREM4"和Vitis vinifera cv. 这两个字的意思是什么? "PN40024"是对日本甲虫 (Popillia japonica) 的草食.
确定"GREM4"中对昆虫草食性抗性有助于形成的形态和遗传因素.

主要方法:

选择和无选择的草食测试进行,以评估甲虫的食偏好和损害.
量化了叶子三体密度,并比较了两个葡萄藤基因型.
进行了比较性转录基因分析,以确定在草食压力下差异表达的基因.

主要成果:

"GREM4"比"PN40024"对日本甲虫食草的耐药性明显更强.
"GREM4"表现出更高的叶子三体密度,甲虫更喜欢有更少三体的地方.
转录组分析显示",GREM4"具有更高的防御和二次代谢物基因的构成表达,以及更广泛的可诱导对草食的反应,富含烯和黄类生物合成途径.

结论:

"GREM4"的昆虫草食耐药性归因于叶子三体和独特的构成性和可诱导性基因表达的组合.
关键的遗传因素包括涉及,黄类,烯类生物合成的基因的增强表达,以及病原体防御途径.

关键词:

维蒂斯·拉布鲁斯卡 (Vitis labrusca) 是一种葡萄树.葡萄酒 (Vitis vinifera) 是一种葡萄酒.进行比较的转录学.黄类化合物黄类化合物昆虫的草食是昆虫的草食日本甲虫日本甲虫烯是一种烯.三合体是三合体的

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