一个新兴的多药耐药blaNDM携带ST182血统的比较基因组学在复杂的Enterobactercloacae中
Angeliki Mavroidi1, Elisavet Froukala2, Athanasios Tsakris2
1Department of Microbiology, Faculty of Medicine, General University Hospital of Patras, 26504 Patras, Greece.
Antibiotics (Basel, Switzerland)
|June 27, 2024
概括
新兴的多药耐药性Enterobacter cloacae复合体 (ECC) ST182菌株正在全球蔓延. 超过一半的人携带了blaNDM基因,这表明他们最近获得了 blaNDM 基因,并构成了需要监测的重大公共卫生威胁.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 肠杆菌cloacae复合体 (ECC) 菌株,特别是E. hormaechei,具有临床意义. 产生碳酶的ECC菌株,通常属于MLST ST114,ST93,ST90和ST78,在医院获得的感染中很普遍. ST182曾经偶尔被报告,但现在正在爆发.
- 最近出现了产生blaNDM的ST182克隆菌株的疫情,突出了对这种特定序列类型的进一步调查的需要.
研究的目的:
- 为了调查ST182在Enterobactercloacae复合体中的流行和演变.
- 探索ST182菌株中blaNDM基因的获取机制.
主要方法:
- 来自4685个E. hormaechei全基因组测序 (WGS) 组件的646个MLSTSTST的家族遗传学分析.
- 在55个ST182WGS组件的in silico比较和基因组学分析.
- 与已发表的等离子体序列的blaNDM-harboring contigs的比较.
主要成果:
- 在2011-2021年间,ST182 E. hormaechei菌株在全球范围内被确定,形成了三个主要的基因组集群.
- 在54.5%的ST182菌株 (30/55) 中,发现了类似于格兰阴性细菌等离子体的blaNDM寄存结构,含有多个抗生素耐药性和毒性基因.
- 在blaNDM-harboring contigs的基因组集群,地理来源或等离子体类型之间没有观察到任何显著的关联.
结论:
- ST182 E. hormaechei菌株是过去十年全球识别的血统.
- 在ST182菌株中多样化的blaNDM遗传结构的高流行率表明最近的收购事件.
- 携带blaNDM的ST182代表了ECC中新兴的多药耐药性和毒性血统,需要密切监控.
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