振动辐射通过破坏其新陈代谢来降低野猪精子质量
Shanpeng Wang1, Xuejun Zeng2,3, Shenao Liu1
1College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
Biology
|June 27, 2024
概括
运输振动对野猪精子质量产生负面影响,减少运动性,膜完整性和ATP水平. 这导致人工授精计划中的生育率和 litter 尺寸下降.
科学领域:
- 动物科学动物科学
- 生殖生物学 生殖生物学
- 生物技术是生物技术.
背景情况:
- 使用液体保存的野猪精液进行人工授精 (AI) 是养猪的标准做法.
- 精液运输涉及潜在的振动暴露,这可能会影响精子质量和生殖结果.
研究的目的:
- 研究模拟运输振动对野猪精子质量和功能的影响.
- 为了确定振动强度和持续时间对精子关键参数和随后的生育能力的影响.
主要方法:
- 猪的射精被集合,稀释,并使用轨道震动器以不同的速度 (0-200rpm) 和持续时间 (0-6h) 进行模拟振动.
- 精子质量通过运动性,血膜和体完整性,线粒体功能 (ATP,ROS,膜潜力),酶活性 (HK,PK,LDH) 和电容性来评估.
- 通过精子结合卵管扩展体和随后的怀孕和人工智能后的 litter 尺寸率来评估生育能力.
主要成果:
- 对振动的暴露显著降低了精子运动性,血膜完整性,体完整性,ATP水平和线粒体功能.
- 振动后观察到pH和线粒体ROS水平的增加,以及抑制的糖解和减少的氨酸酸化.
- 暴露于振动的精液导致精子-卵巢结合的减少,怀孕率降低, litter 尺寸更小.
结论:
- 运输振动会对野猪的精子质量和功能产生不利影响,影响生育能力.
- 振动压力会损害精子的运动性,能量代谢和容量,导致生殖成功减少.
- 在精液运输过程中减轻对振动的暴露对于保持猪养殖人工智能的有效性至关重要.
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