在牛基因型 (L. Walp) 中的遗传异质性使用DArTseq (GBS) 衍生单核酸多形态
Goitsemang Mahlomola Hendry Dikane1, Moosa Mahmood Sedibe1
1Department of Agriculture, Faculty of Health and Environmental Sciences, Central University of Technology, Private Bag x20529, Bloemfontein 9301, South Africa.
Genes
|June 27, 2024
概括
这项研究使用多样性阵列技术 (DArTseq) 评估牛基因型多样性单核酸多态 (SNP). 关键的基因型被确定为异质的,支持牛繁殖计划和生殖质管理.
科学领域:
- 农业科学 农业科学
- 遗传学 是一个遗传学.
- 植物育种 植物育种
背景情况:
- 牛 (Vigna unguiculata) 是重要的食物和料作物,具有悠久的历史.
- 了解遗传多样性对于改善豆品种至关重要.
研究的目的:
- 为了评估92种牛基因型的遗传异质性.
- 用高通量单核酸多态 (SNP) 来进行多样性评估.
主要方法:
- 使用CTAB协议从14天老的牛豆叶中提取DNA.
- 使用多样性阵列技术 (DArTseq) 进行的基因型鉴定.
- 使用R软件 (vcfR,poppr,ape) 分析了16960个过的SNP.
主要成果:
- 已经确定了特定的异质牛豆基因型:TVU 9596,Orelu,90K-284-2,RV 403和RV 498.
- 遗传多样性指标的平均值,包括观察到的异构性 (0.386) 和预期的异构性 (0.345).
- 计算了多态信息含量 (0.345) 和内生系数 (0.113).
结论:
- DArTseq方法有效地揭示了牛基因型中显著的遗传多样性.
- 研究结果支持在未来的牛繁殖计划中使用这些多样化的基因型.
- 经过验证的遗传多样性有助于有效的牛生殖质管理.
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