生物物理因素在器官发育中的作用:来自当前有机体模型的见解
Yofiel Wyle1,2, Nathan Lu1, Jason Hepfer1
1Department of Surgery, School of Medicine, University of California-Davis, Sacramento, CA 95817, USA.
Bioengineering (Basel, Switzerland)
|June 27, 2024
概括
诸如组织硬等生物物理因素对人类胚胎发育至关重要. 将这些机械力集成到有机体模型中,可以提高它们对再生医学的临床相关性.
科学领域:
- 生物物理学的生物物理.
- 发展生物学 发展生物学
- 再生医学是一种再生医学.
背景情况:
- 传统的体外器官生成模型忽视了机械力,只关注生化线索.
- 标准的细胞培养基质 (聚乙烯) 比原生人体组织 (kPa范围) 显著更硬 (GPa范围).
- 器官技术迅速发展,创造了干细胞衍生组织,在转录上类似于胎儿器官.
研究的目的:
- 审查生物物理因素在人类胚胎发育和器官生成中的作用.
- 突出目前体外模型的局限性和有机体的潜力.
- 建议将机械参数整合到有机体模型中,以提高生理学相关性和治疗应用.
主要方法:
- 审查现有的关于有机体发生和有机体发育中的生物物理因素的文献.
- 讨论组织特性,如刚性,粘性弹性和剪切力.
- 探索Hippo-YAP/TAZ信号通路在机械传导中的作用.
主要成果:
- 生物物理因素,包括组织刚性,粘弹性和剪切力,对于器官形成和疾病至关重要.
- 有机体,模仿本地胎儿组织,显示出胎儿再生医学和药物开发的希望.
- 希波-YAP/TAZ通路调解了细胞外矩阵刚性和细胞力学之间的相互作用.
结论:
- 纳入生物物理线索的有机体模型将提高生理学相关性和治疗适用性.
- 在胎儿再生医学中,有机体已经准备好早期临床影响.
- 未来的应用可能会利用先进的物理学,如量子纠,来理解生物过程,如DNA突变.
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