相关实验视频

Updated: Jun 22, 2025

Author Spotlight: Unraveling Plant Responses to Abiotic Stresses Using the PlantScreen Robotic Platform

Author Spotlight: Unraveling Plant Responses to Abiotic Stresses Using the PlantScreen Robotic Platform

Published on: June 7, 2024

热应激和微生物应激诱导了药用植物中的防御性醇积累Sparganium stoloniferum

Mengru Sang^1,2,3, Qinan Liu⁴, Dishuai Li³

¹Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China.

International journal of molecular sciences

|June 27, 2024

概括

药用草药Sparganium stoloniferum在热量和微生物压力下积累生物活性,增强其化学防御. 这种反应涉及特定的基因和蛋白质,为培养高含量品种提供了洞察力.

科学领域:

植物科学植物科学
生物化学生物化学
微生物学微生物学

背景情况:

像Sparganium stoloniferum (SL) 这样的药用植物产生生物活性.
在SL中的生物合成与植物病原体相互作用基因有关.
在SL生长过程中观察到环境压力,如热量和微生物存在.

研究的目的:

研究在压力下SL中生物活性积的调节和机制.
了解热和微生物应激在SL的生产中的作用.
确定参与生物合成的关键基因和蛋白质.

主要方法:

整合LC-MS/MS分析,16S rRNA测序,以及RT-qPCR.
分子测试用于研究压力下的类代谢物生物合成.
防御性蛋白质的识别和验证 (Ss4CL,SsC4H,SsF3'5'H).

主要成果:

用于生物合成的代谢物和基因都在热量或Pseudomonas细菌压力下升级.
含量和基因表达表明,SL的基化学防御受到了刺激.
快速的积与氨基酸消耗有关,并确定了关键的防御蛋白质.

结论:

SL中的生物活性是环境应激反应的结果,增强了它的化学防御.
这项研究阐明了在压力下积累的机制.
这些发现为培育含含量高的SL提供了洞察力.

关键词:

斯帕尔加尼乌姆 (Sparganium stoloniferum) 是一种的植物.氨基酸氨基酸是一种氨基酸.化学防御的化学防御热应激是一种热应激.微生物压力是微生物的压力.醇是一种醇.

更多相关视频

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

Published on: October 11, 2024

相关概念视频

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

Responses to Drought and Flooding

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.

Responses to Salt Stress

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.

Adaptations that Reduce Water Loss

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.

Regulation of Transpiration by Stomata

Regulation of Transpiration by Stomata

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.