维生素D和硫福拉降低炎症氧化应激,并恢复表皮完整性的标记物在与年龄相关的黄斑退化在体外模型中
Loredana Bergandi1, Giulia Palladino1, Alessandro Meduri2
1Department of Oncology, University of Torino, Via Santena 5 bis, 10126 Torino, Italy.
International journal of molecular sciences
|June 27, 2024
概括
维生素D和硫福拉可以对抗与年龄相关的黄斑变性 (AMD) 相关的氧化应激和炎症. 这些化合物通过减少有害分子和恢复屏障完整性来保护视网膜细胞,这表明潜在的AMD疗法.
科学领域:
- 眼科医生 眼科 眼科
- 细胞生物学 细胞生物学
- 营养科学 营养科学
背景情况:
- 与年龄相关的黄斑变性 (AMD) 病原包括慢性氧化应激,炎症和表皮屏障功能受损.
- 异常的新血管化是晚期AMD的标志,导致视力丧失.
研究的目的:
- 研究维生素D (VD) 和硫 (SF) 在缓解AMD相关的细胞损伤方面的治疗潜力.
- 阐明VD和SF对视网膜上皮细胞转化生长因子β (TGF-β) 诱导的炎症的保护作用的分子机制.
主要方法:
- 用TGF-β刺激ARPE-19视网膜上皮细胞以模拟AMD相关的炎症.
- 用VD和/或SF对细胞进行治疗,以评估它们对活性氧物种 (ROS) 生产,促炎性细胞因子表达,线粒体呼吸,血管内皮生长因子 (VEGF) 分泌和上皮完整性标记物的影响.
主要成果:
- VD和SF显著降低了TGF-β诱导的ROS水平,以及插白素-6和插白素-8的表达.
- 治疗阻止了线粒体呼吸系统元素的TGF-β介导上调,并降低了VEGF分泌.
- 联合VD和SF治疗显示出潜在的效果,通过ERK1/2通路恢复上皮质完整性标记物,独立于VD受体表达.
结论:
- 在AMD的细胞模型中,维生素D和硫福拉表现出强大的协同抗炎和细胞保护作用.
- 这些化合物有效地抵消了AMD的关键病理特征,包括氧化应激,炎症和血管信号.
- 这些发现支持进一步对VD和SF进行临床研究,以作为管理与年龄相关的黄斑退化症的联合治疗策略.
关键词:
人类视网膜色素上皮细胞这是一种炎症炎症炎症炎症.有活性氧物种的反应性氧物种.硫甲是一种硫甲.转化生长因子-β (TGF-β) 的转化生长因子血管内皮生长因子 (VEGF)维生素D受体是维生素D的受体.更多相关视频
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