肠道生成和透性 肠道生成和透性
Anna Casselbrant1, Erik Elias1, Peter Hallersund1
1Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy at the University of Gothenburg, 40530 Gothenburg, Sweden.
International journal of molecular sciences
|June 27, 2024
概括
高脂肪饮食可能会增加肠道的透性,但健康的人的肠道适应短期的变化. 体在实验室研究中影响肠道细胞,但在高脂肪和高碳水化合物饮食的人群中没有显著的影响.
科学领域:
- 胃肠病学 胃肠病学
- 营养科学 营养科学
- 分子生物学分子生物学
背景情况:
- 高脂肪饮食 (HFD) 与增加肠道透性和全身炎症有关.
- HFD可以诱导线粒体3-基-3-甲基氨基-CoA合成酶 (HMGCS) 的阴茎表达,这是体合成中的关键酶.
- 肠道生成和体在调节肠道屏障功能的作用在很大程度上是未知的.
研究的目的:
- 研究高碳水化合物饮食与高碳水化合物饮食对人类肠道炎症,营养感应和透性的影响.
- 探索体调节肠道屏障功能的假设,可能是通过紧密结合蛋白.
主要方法:
- 人类研究:15名健康志愿者接受了为期2周的高碳水化合物饮食与高碳水化合物饮食,评估炎症标志物和内毒性血症.
- 对蛋白质表达 (西式斑点,免疫组织化学) 和紧密结合形态 (电子显微镜) 进行分析.
- 试验室/活体外研究:使用Ussing腔室分析透性和生成的Caco-2细胞,小鼠和人类肠体.
主要成果:
- 人类的饮食之间没有炎症标志物或食后内毒素的显著差异.
- 在体外/体外:体通过PPARα对HMGCS呈现负面反,并影响了肠粘膜的透性.
- 在人体体内进行的研究:没有观察到紧结蛋白或透性的变化;体抑制增加了粘膜抵抗.
结论:
- 虽然体可以在体外/体外调节肠道细胞,但2周的HFD在健康人群中没有增加肠道透性.
- 健康的人类肠道似乎能够适应巨量营养素的极端和内源体的增加.
- 除了饮食成分之外的因素可能是必要的,以诱导肠道透性 in vivo.
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