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在外源性黑色素治疗下,类水果中黄积累的综合代谢-转录组分析

Chenning Zhao¹, Zhendong Wang¹, Zhenkun Liao¹

¹Laboratory of Fruit Quality Biology, Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou 310058, China.

International journal of molecular sciences

|June 27, 2024

概括

黑色素治疗通过改变基因表达来增强特定的类黄 (PMF),同时减少其他类型的类黄. 这种激素可以通过昼夜节律调节来影响类黄路径.

科学领域:

植物生物化学植物生物化学
激素信号传递激素信号传递
果物新陈代谢果物新陈代谢

背景情况:

类水果中的黄类化合物具有重要的制药价值.
一种植物激素 - - 黑色素 (melatonin) 影响植物的发育和应激反应,可能会影响黄酸的积累.

研究的目的:

为了研究外源性黑激素对"Bingtangcheng"类水果的黄类生物合成的直接影响.
阐明类水果新陈代谢中由激素诱导的变化背后的分子机制.

主要方法:

使用非目标代谢学和转录学来分析类水果的变化.
基因表达分析确定了关键的酶和转录因子,这些酶和转录因子参与了黄路径.
皮尔森相关性分析将转录因子与差异积累的黄酸联系起来.

主要成果:

黑色素治疗显著增加了聚甲化黄 (PMFs) 和调高了关键的黄类生物合成基因 (例如,4CL,FNS,FHs).
基合成酶 (CHS) 的下调,导致减少了黄类糖化物含量.
转录因子,特别是AP2/EREBP成员,与改变的黄类蛋白水平共同表达. 昼夜节律和光合作用途径被不同表达的基因丰富.

结论:

外源性黑激素调节类黄类生物合成,使PMFs优于糖化物.
黑素的作用可能涉及调节特定的黄类通路基因和转录因子.
黑素可能通过对昼夜节律和光合作用的影响影响类黄代谢.

关键词:

类的类植物.黄类化合物黄类化合物黑激素是什么意思黑激素是什么意思代谢组代谢组的代谢监管监管监管监管监管监管转录组 (transcriptome) 是一个转录组.

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