在胆囊癌细胞中,LINC00662通过miR-335-5p调节OCT4表达来促进侵略性特征
Pablo Pérez-Moreno1, Ismael Riquelme2, Carolina Bizama3
1Programa de Comunicación Celular en Cáncer, Facultad de Medicina Clínica Alemana, Universidad del Desarrollo, Santiago 7780272, Chile.
International journal of molecular sciences
|June 27, 2024
概括
长跨基因非蛋白编码RNA 662 (LINC00662) 通过促进干性,入侵性和化学抵抗性来驱动胆囊癌的攻击性. 这项研究揭示了LINC00662作为胆囊癌的潜在生物标志物和治疗点.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 遗传学 是一个遗传学.
背景情况:
- 长非编码RNAs (lncRNAs) 是生物过程和疾病的关键调节者.
- LINC00662与各种癌症有关,但其在胆囊癌中的作用仍然未被探索.
- 了解 lncRNA 功能对于癌症研究至关重要.
研究的目的:
- 为了研究LINC00662在胆囊癌 (GBC) 攻击性中的作用.
- 确定LINC00662是否有助于GBC中的茎状表型,入侵和化学抵抗.
- 阐明LINC00662在GBC中的功能背后的分子机制.
主要方法:
- 在GBC患者样本中分析LINC00662表达.
- 对干细胞标记物 (CD133,CD44) 和干性基因的评估.
- 评估侵袭性能力和表皮层-介质细胞过渡 (EMT) 标志物.
- 化疗耐药性测定使用西斯和5-甲.
- 研究涉及microRNA 335-5p (miR-335-5p) 和OCT4.4的调节途径.
主要成果:
- 在GBC患者中,LINC00662表达与较大的瘤大小和淋巴结转移相关.
- 过度表达LINC00662会增加CD133+/CD44+细胞群和干细胞基因表达.
- LINC00662增强了GBC细胞的入侵,并促进了EMT.
- 通过调节ABC输送器,LINC00662赋予了对西斯普拉丁和5-甲的耐药性.
- LINC00662通过降低 miR-335-5p 的调节和提高 OCT4.4 的调节而起作用.
结论:
- LINC00662显著促进胆囊癌的攻击性,促进干性,入侵性和化学抵抗性.
- LINC00662/miR-335-5p/OCT4轴是推动GBC进展的关键机制.
- LINC00662 作为预后不佳的潜在生物标志物和GBC中的治疗点.
相关概念视频
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Induced Pluripotent Stem Cells
4.0K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.0K
lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K


