植物酶不需要蛋白质分解活性来实现其压力诱导的内部化
Tatevik A Torosian1, Anastasia I Barsukova1, Nina V Chichkova2
1Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow 199991, Russia.
International journal of molecular sciences
|June 27, 2024
概括
在压力期间,植物细胞中的植物酶重新内化不需要蛋白质分解活性. 这一发现揭示了酶的作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 蛋白酶的功能和调节.
- 细胞应激反应细胞应激反应
背景情况:
- 酸酶是一种独特的植物细素样蛋白酶家族,表现出酸盐裂解特异性和动态局部化.
- 这些蛋白酶由健康的植物细胞分泌,但在应激感知时重新内化.
研究的目的:
- 调查植物酶的蛋白质分解活性是否对其压力诱导的逆向转移至关重要.
- 为了阐明植物酶重新定位的机制,以应对细胞压力.
主要方法:
- 在 *Nicotiana benthamiana* 叶细胞中采用了跨补充方法.
- 表达了一种具有自由原体的无原体植物酶 (NtPhyt),以实现活性酶生成和分泌.
- 产生了NtPhyt的催化不活跃突变物 (Ser537Ala),以评估蛋白质分解活性在运输中的作用.
主要成果:
- 成功生成了蛋白分解活性的NtPhyt,并实现了与原生植物酶相比的细胞外传输.
- 不活跃的NtPhyt Ser537Ala突变体被分泌,但在氧化应激诱导时保留了逆向运输的能力.
- 证明经过处理,但不活跃的植物酶可以重新内化到植物细胞中.
结论:
- 植物酶的蛋白质分解活性对于其应激诱导的逆行运输是不可或缺的.
- 在压力期间,植物酶的重新内化机制并不依赖于它的酶功能.
- 这项研究阐明了植物中植物酶局部化动力学和应激反应的一个关键方面.
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