在潮湿的亚热带气候下在咖啡加工过程中探索微生物对风味发展的影响,通过转基因-代谢学分析
Alexander da Silva Vale1, Cecília Marques Tenório Pereira2, Juliano De Dea Lindner2
1Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), Curitiba 81530-900, PR, Brazil.
Foods (Basel, Switzerland)
|June 27, 2024
概括
这项研究探讨了咖啡加工中的微生物相互作用,揭示了影响味道的关键细菌和真菌. 研究结果表明,受控发酵可以在亚热带气候下提高咖啡质量.
科学领域:
- 微生物生态学 微生物生态学
- 食品科学 食品科学 食品科学
- 农业科学 农业科学
背景情况:
- 微生物相互作用对于咖啡的风味和弹性至关重要.
- 气候变化需要在咖啡生产中采取适应性战略.
- 了解微生物在发酵中的作用对于提高质量至关重要.
研究的目的:
- 研究微生物社区的动态和咖啡加工中的风味贡献.
- 在潮湿的亚热带气候下分析微生物相互作用和代谢物生产.
- 确定关键的微生物物种及其对咖啡风味特征的影响.
主要方法:
- 用于微生物多样性分析的illumina测序.
- 高性能液体染色学 (HPLC) 和气体染色学-质谱学 (GC-MS) 用于代谢物分析.
- 网络分析用于绘制微生物相互作用和物质相关性的地图.
主要成果:
- 确定了主要的原生生物 (Enterobacter,Erwinia) 和真核生物 (Fusarium,Cladosporium) 微生物.
- 主要代谢物包括乙醇,乳酸,酸和酸.
- 细菌群落通过和酒精生产显著影响口味;咖啡因和林纳醇保持稳定.
- 网络分析显示了321个相互作用,其中Enterobacter,Kluyvera和Serratia与糖和挥发性化合物有关.
结论:
- 微生物适应亚热带条件的影响咖啡发酵和质量.
- 控制的发酵实践提供了增强咖啡风味和一致性的策略.
- 这些发现有助于理解环境变化下的食品发酵中的微生物生态.
相关概念视频
Microbial Fermentation
1.8K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.8K
Methods to Assess Microbial Communities
60
Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...
60
Microbes in Food Production
403
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
403
Microbes in Beverage Production
298
Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
298
Microbes in the Production of Fermented Foods
327
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
327
Development of Human Microbiota
61
The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from...
61


