拉曼激活细胞喷射用于验证Raman食源病原体指纹数据库的可靠性
Shuaishuai Yan1,2, Xinru Guo1, Zheng Zong1
1College of Food Science, Shanxi Normal University, Taiyuan 030031, China.
Foods (Basel, Switzerland)
|June 27, 2024
概括
拉曼光谱与机器学习相结合,可以准确识别食物传播的病原体. 这种方法将表型拉曼指纹与基因型识别联系起来,确保快速检测病原体的可靠数据库.
科学领域:
- 微生物学 微生物学
- 频谱学是一种光谱学.
- 生物信息学是一种生物信息学.
背景情况:
- 快速识别食源性病原体对于公共卫生和食品安全至关重要.
- 使用拉曼光谱的表型鉴定需要可靠的基因型确认,以确保数据库的准确性.
研究的目的:
- 开发和验证基于拉曼光谱的方法,用于快速识别食源性病原体.
- 通过关联表型和基因型数据来确定拉曼指纹数据库的可靠性.
主要方法:
- 使用t分布式静态邻居嵌入 (t-SNE) 和支持向量机 (SVM) 的分类模型被开发用于病原体识别.
- 拉曼激活细胞喷射 (RACE) 用于隔离已识别的细菌.
- 进行了单细胞基因组DNA放大和测序以进行物种分析.
主要成果:
- 该分类模型在四种食源性病原体中实现了97.04%的识别精度.
- 预测表型与实际基因型匹配的准确性至少为83.3%.
- 所有预测的测序结果与已识别的物种相对应.
结论:
- 拉曼光谱与机器学习相结合,提供了一种可靠的方法来快速检测食物传播病原体.
- 开发的拉曼指纹数据库,通过基因型分析验证,对食品安全应用很有前途.
相关概念视频
01:20Raman Spectroscopy: Overview
355
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
355
01:26Raman Spectroscopy Instrumentation: Overview
324
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
324