工业食品生产废物提取物对病原性细菌的抗菌活性潜力:比较分析和表征
James Ziemah1, Matthias S Ullrich1, Nikolai Kuhnert1
1School of Science, Constructor University Bremen, 28759 Bremen, Germany.
Foods (Basel, Switzerland)
|June 27, 2024
概括
这项研究调查了食品生产废物作为生物活性化合物的来源,发现皮,热和咖啡银皮提取物具有显著的抗菌和抗氧化特性,没有观察到细胞毒性. 这些发现突出了食品废弃物的可持续工业应用潜力.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物技术是生物技术.
- 可持续化学 可持续化学
背景情况:
- 显著的粮食生产浪费 (估计损失17%) 需要可持续的解决方案.
- 对工业食品废弃物的生物活性化合物的利用进行了有限的调查.
- 食品废弃物提取物的潜在健康和工业应用尚未得到充分探索.
研究的目的:
- 探索皮 (LP),热 (HT) 和咖啡银皮 (CSS) 提取物作为生物活性化合物的来源的潜力.
- 为了评估这些食品废弃物提取物的抗菌,抗氧化和抗糖尿病特性.
- 为了描述提取物的化学成分.
主要方法:
- 用水合甲醇提取LP,HT和CSS.
- 对抗阳性和阴性细菌的抗菌活性进行格扩散试验.
- 最低抑制度 (MIC) 的确定.
- 总含量 (TPC) 分析.
- 使用ABTS和DPPH测定进行抗氧化活性评估.
- 对HaCaT细胞的细胞毒性评估.
- 胰腺氨酶抑制测定对抗糖尿病潜力的测定.
- 液体染色学-质谱学 (LC-MS) 用于化合物识别.
主要成果:
- 提取产量:LP (482毫克/克) >HT (332毫克/克) >CSS (20毫克/克).
- 所有的提取物都显示出对测试的病原体具有广泛的抗菌活性.
- 在LP和CSS提取物中观察到高抗氧化活性.
- 显著的胰腺粉酶抑制 (92%在LP中),表明抗糖尿病潜力.
- 对HaCaT细胞没有细胞毒性作用.
- 在LC-MS中确定了多 (CSS),化化合物 (HT) 和黄 (LP).
结论:
- 食品生产废物 (LP,HT,CSS) 是生物活性化合物的丰富来源.
- 这些提取物具有显著的抗菌,抗氧化剂和潜在的抗糖尿病特性.
- 这些发现支持在各种工业应用中可持续利用食品废弃物.
相关概念视频
Microorganisms in Agriculture and Food industry
Microorganisms play a crucial role in agriculture and the food industry, contributing to soil fertility, crop protection, and food production. Their functions range from nitrogen fixation and biopesticide production to fermentation and food preservation, making them indispensable to sustainable farming and food safety.Role in AgricultureNitrogen-fixing bacteria, such as Rhizobium (symbiotic) and Azotobacter (free-living), convert atmospheric nitrogen into ammonia through biological nitrogen...
Biological Methods for Microbial Control
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
Antimicrobial Effectiveness
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
Sources of Food Contamination
Contamination of food by microbial agents and natural toxins poses significant risks to public health. These hazards can be introduced at various points across the food supply chain, ranging from environmental sources to processing and storage stages. Understanding these contamination pathways is critical for developing strategies to ensure food safety.Seafood is particularly vulnerable to contamination through both environmental exposure and microbial colonization. Toxins from harmful algal...
Production of Antibiotics
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
Clinical Significance of Antibiotic Resistance
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...


