在用益生菌治疗的临床不育患者的阴道微生物群下一代测序分析的试点研究
Li-Te Lin1,2,3, Chia-Jung Li1,2, Chia-Chun Wu1
1Department of Obstetrics and Gynaecology, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan.
Journal of clinical medicine
|June 27, 2024
概括
口服益生菌可能会改变接受辅助生殖技术 (ART) 的女性的阴道微生物群. 补充表明加德内雷拉减少,乳酸菌和双菌增加,可能改善细菌简介,以获得更好的生殖结果.
科学领域:
- 生殖医学 生殖医学
- 微生物组研究 微生物组研究
- 胃肠病学 胃肠病学
背景情况:
- 阴道微生物组在生殖成功中起着至关重要的作用.
- 了解干预措施对阴道微生物群的影响对于辅助生殖技术 (ART) 患者至关重要.
- 正在探索口服益生菌补充剂对阴道环境的潜在影响.
研究的目的:
- 调查口服益生菌补充剂对阴道微生物群组成的影响.
- 在为ART做准备的妇女服用益生菌后,评估细菌丰度和比率的变化.
- 探索益生菌对改善这种人口群体阴道细菌特征的潜在益处.
主要方法:
- 招募了30名接受ART治疗的女性 (31-43岁).
- 分析了使用16S核糖体RNA (rRNA) 测序的阴道微生物组成.
- 在服用口服益生菌补充剂之前和之后,比较了微生物群的特征.
主要成果:
- 确定了17种微生物,包括8种乳酸菌种.
- 观察到Gardnerella减少了约20%,Lactobacillus增加了约10%,Bifidobacterium增加了约15%.
- 补充剂后注意到Firmicutes/Bacteroidetes (F/B) 比率的显著降低.
结论:
- 口服益生菌可以诱导阴道微生物群的显著变化中年妇女接受ART.
- 这些发现表明,总体细菌特征可能有所改善,需要进一步调查.
- 未来的研究应该集中在更大的队列,更窄的年龄范围和荷尔蒙因素上,以验证和深化洞察力.
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