开发一种植物表达的抗牛病亚单元疫苗
Daria A Rutkowska1, Lissinda H Du Plessis2, Essa Suleman1
1Advanced Agriculture and Food Cluster, Council for Scientific and Industrial Research, Pretoria 0001, South Africa.
Microorganisms
|June 27, 2024
概括
在开发一种新的乳病疫苗时,研究人员设计了植物性类似病毒的颗粒,显示Brucella melitensis表现. 一个模拟粒子在小鼠中证明了安全性和保护性有效性,为新型子单位疫苗铺平了道路.
科学领域:
- 疫苗学 疫苗学 疫苗学
- 植物生物技术 植物生物技术
- 免疫学 免疫学 免疫学
背景情况:
- 兽是一种重要的动物传染病,目前的疫苗存在安全风险.
- 现有的布鲁塞拉疫苗是有效的,但导致动物流产,对人类是致病的.
- 病毒样颗粒 (VLP) 为免疫系统呈现抗原提供了一个有前途的支架.
研究的目的:
- 开发一种新,安全和有效的针对牛病的亚单元疫苗.
- 在Orbivirus核状颗粒 (CLPs) 上呈现Brucella melitensis T细胞表位.
- 在Nicotiana benthamiana植物中表达化学CLP,用于潜在的疫苗生产.
主要方法:
- 精选的Brucella melitensis T细胞表位被设计到Orbivirus核心样粒子 (CLPs) 上.
- 化学CLP在Nicotiana benthamiana植物中被暂时表达.
- 植物表达的仿真CLP的安全性和有效性在小鼠brucellosis模型中进行了评估.
主要成果:
- 在N. benthamiana.中成功组装了五种不同的仿真CLP,每种都显示出独特的T细胞表位.
- 在BALB/c小鼠中测试了三种高产的仿真CLP,并发现它们在BALB/c小鼠中安全.
- 一种仿真CLP给予了对毒性布鲁塞拉疫苗的保护,与商业Rev1疫苗相当.
结论:
- 可以成功地组装出植物表达的含有Brucella melitensis T细胞表位体的仿真CLP.
- 开发的仿真CLP在小鼠模型中是安全的.
- 这项研究是迈向安全有效的植物性布鲁塞洛斯亚单位疫苗的关键第一步.
关键词:
布鲁塞拉病毒是什么?在 CLP 中,我们可以使用 CLP这种病毒是Orbivirus.类似于核心的粒子.标志性 标志性 标志性 标志性新一代的新一代.植物表达的植物表达.这是一个子单位子单位.疫苗 疫苗 疫苗 疫苗更多相关视频
00:10Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation
Published on: August 21, 2019
7.5K
12:53Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development
Published on: March 16, 2022
1.8K
相关概念视频
Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Microorganisms in Medicine and Therapeutics
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
Vaccine Production
Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...
