猪大肠杆菌病:肠道细菌微生物组的分析
Wanli Sha1,2, Emad Beshir Ata3, Man Yan2
1Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Microorganisms
|June 27, 2024
概括
具有肠毒性E.E. 大肠杆菌感染扰乱了猪肠道微生物群,并导致大肠杆菌病理变化. 恢复导致独特的微生物菌群,有助于控制猪大肠杆菌病爆发.
科学领域:
- 兽医微生物学 兽医微生物学
- 动物科学动物科学
- 肠道微生物组研究研究
背景情况:
- 肠毒性大肠杆菌 (E. coli) 是一种有毒的物质. coli) 引起小猪的重大疾病,导致腹和猪生产的经济损失.
- 了解E.E.的影响 对猪肠道微生物群和肠道健康的影响对于制定有效的控制策略至关重要.
研究的目的:
- 为了研究干扰猪肠道微生物群的组成和结构后的肠毒性E. 大肠杆菌感染.
- 为了评估感染E.E.的小猪肠道组织的组织病理变化. 大肠杆菌.
主要方法:
- 从腹,康复和健康的小猪中收集了便样本,用于16S rRNA基因测序,以分析肠道微生物群.
- 对肠道组织进行了检查,以检查其病理学变化.
- 操作分类单位 (OTU) 和主要坐标分析 (PCoA) 用于评估微生物丰富性和社区结构.
- 线性差异分析效果大小 (LEfSe) 用于识别差异丰富的种类.
主要成果:
- 主要观察到的基因病理变化主要是在受感染的小猪的皮质上.
- 这个E.E. 大肠杆菌和腹组显示了最高的细菌丰富度,表明显著的肠道失调.
- PCoA显示出明显的微生物聚类,恢复组与健康和感染组相比,呈现出独特的肠道菌群.
- 主导类包括Bacteroidetes,Firmicutes和Fusobacteria;然而,LEfSe在恢复的小猪和Muribaculacea家族丰富中识别了像Euryarchaeota和Bacteroidota这样的特定类.
结论:
- 具有肠毒性E.E. 大肠杆菌感染会诱导小猪的显著肠道失生症和状细胞病理损伤.
- 猪肠道微生物组合在感染和恢复期间发生变化,每个状态都有不同的微生物特征.
- 这些发现为猪大肠杆菌病期间的微生物组动态提供了宝贵的见解,有助于制定针对疫情控制的有针对性的干预措施.
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