氨基面菌 (Bacillus amyloliquefaciens) LM-1 影响Magnaporthe oryzae中的多个细胞生物过程,以抑制大米爆发
Meiling Liang1, Aiqing Feng1, Congying Wang1
1Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute of Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Microorganisms
|June 27, 2024
概括
杏仁菌菌株 LM-1 菌株有效控制由Magnaporthe oryzae引起的米爆病. 这种生物控制剂破坏了真菌细胞的完整性,化和压缩物形成,为作物保护提供了一种新的策略.
科学领域:
- 植物病理学 植物病理学
- 微生物学 微生物学
- 生物控制生物控制
背景情况:
- 由Magnaporthe oryzae引起的水爆发是对全球水生产的重大威胁.
- 氨基面菌是一种已知的生物控制剂,但其对抗大米爆炸的机制尚未完全阐明.
研究的目的:
- 为了识别和表征Bacillus amyloliquefaciens菌株 (LM-1),用于对大米爆发的生物控制.
- 研究LM-1抑制Magnaporthe oryzae生长和致病性的特定机制.
主要方法:
- 生物控制菌株LM-1的隔离和鉴定使用形态,生化和16S rDNA测序.
- 与LM-1和M. oryzae进行共同培养实验,光显微镜 (calcofluor白色染色).
- 对M. oryzae appressorium形成,状细胞死亡,自和大米细胞反应 (ROS,铁积累) 的LM-1超效应的分析.
主要成果:
- LM-1被确定为Bacillus amyloliquefaciens,对M. oryzae和Bipolaris oryzae表现出抑制作用.
- LM-1 破坏了 M. oryzae 菌的完整性,抑制了 conidia 生产,并干扰了 appressorium 形成.
- 来自LM-1的超剂减少了自细胞的降解,并诱导了过敏反应,ROS的产生,并减少了在米细胞中积累的铁,而不会损害种子发芽.
结论:
- 巴西氨酸菌株菌株LM-1显示出对抗大米爆发的显著生物控制潜力.
- LM-1通过损害M. oryzae细胞壁完整性,化和压缩体发育以及调节植物防御反应来发挥其作用.
- 这项研究提供了关于B. amyloliquefaciens在控制大米爆炸中的多方面的机制的见解,为可持续农业实践铺平了道路.
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