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相关概念视频

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

587
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
587
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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Centrifugation01:05

Centrifugation

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Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
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Subcellular Fractionation01:32

Subcellular Fractionation

7.0K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
7.0K

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Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration
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一个高面积比率决定性的侧向位移阵列,用于高通量分成.

Jonathan Kottmeier1, Maike S Wullenweber2,3, Ingo Kampen2,3

  • 1Institute of Microtechnology, TU Braunschweig, 38124 Braunschweig, Germany.

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概括

确定性横向位移 (DLD) 装置现在可以在高吞吐量时分成微粒. 这一突破使化学,制药和回收技术的新工业应用成为可能,通过实现近1L/hr的流量.

关键词:
确定性的横向位移 (DLD)高的高面比,高的高面比.高吞吐量,具有高吞吐量.微流体学 在微流体学方面取决于大小的分成部分.

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科学领域:

  • 微流体学 微流体学
  • 生物技术是生物技术.
  • 化学工程是化学工程的重要组成部分.

背景情况:

  • 使用确定性横向位移 (DLD) 的微粒子分化工业应用受到低吞吐量的限制.
  • 高体积流量需要研究DLD设备中的高流速和侧面比.
  • 缺乏关于在雷诺兹数高于60和面积比高于4:1的10微米以下颗粒的DLD分离的实验数据.

研究的目的:

  • 通过使用确定性横移 (DLD) 装置来研究高吞吐量微粒子分化.
  • 开发和测试具有高比例的DLD设备,以提高流速.
  • 为化学,制药和回收行业提供新的工业应用.

主要方法:

  • 开发了一种微制造工艺,使用深度反应性离子蚀刻和高比例 (6:1) DLD设备的阳极粘合.
  • 利用模拟和实验分成的聚乙烯颗粒悬浮物.
  • 在高达15毫升/分钟 (雷诺德数高达90) 的流量下研究了颗粒分离.

主要成果:

  • 成功制造并测试了具有6:1比例的DLD设备.
  • 在高流速 (高达Re=90) 实现了2μm与10μm和5μm与10μm颗粒混合物的成功分离.
  • 实现了前所未有的微粒子分化吞吐量,即近1L/hr.

结论:

  • 高比例的DLD设备可以实现显著更高的微粒分成的吞吐量.
  • 开发的DLD技术克服了以前的局限性,为工业规模的应用铺平了道路.
  • 这一进步为化学,制药和回收行业开辟了新的可能性.