DEK219和HSF17共同调节玉米中核的长度.
Sidi Xie1, Ran Tian1, Hanmei Liu2
1State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China.
Plants (Basel, Switzerland)
|June 27, 2024
概括
对于产量至关重要的玉米核长度由DEK219.9规范. 这项研究确定了热冲击转录因子17 (HSF17) 作为负调节剂,抑制DE18并影响内核长度.
科学领域:
- 植物遗传学和分子生物学
- 农作物科学 农作物科学
- 玉米 (Zea mays L.) 的研究.
背景情况:
- 玉米核长度是决定玉米产量的关键因素.
- 关于控制玉米核长度的遗传机制的知识有限.
- 玉米突变缺陷内核219 (dek219),编码DICER-LIKE1,影响miRNA生物发生和内核长度.
研究的目的:
- 调查DEK219在调节玉米核长度中的作用.
- 为了确定参与DEK219介导的核长度控制的基因和转录因子.
- 阐明DEK219影响内核发展的分子机制.
主要方法:
- 对dek219突变体的表型分析.
- 预测miRNA目标基因,表达分析和相关性分析.
- 过时表达分析和电泳性移动性转移试验 (EMSA).
主要成果:
- 突变的dek219始终显示了减少的内核长度.
- 在DEK219控制下,九个转录因子被确定为潜在的调节因子.
- 热冲击转录因子17 (HSF17) 显著上调,并被确定为负调节剂,抑制DEFECTIVE ENDOSPERM18 (DE18) 表达,导致hsf17突变体内核长度增加.
结论:
- DEK219在玉米核长度调节中发挥作用,可能通过调节转录因子活性.
- HSF17通过抑制DE18起到对玉米长度的负调节作用.
- 这项研究提供了对玉米核发育的见解,并为培育改进品种提供了遗传资源.
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