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微型化工发光系统用于在水中检测ATP

Giuseppe E Capuano¹, Domenico Corso¹, Roberta Farina^1,2

¹Istituto per la Microeletttronica e Microsistemi-Consiglio Nazionale delle Ricerche, VIII Strada Z.I., 5, 95121 Catania, Italy.

Sensors (Basel, Switzerland)

|June 27, 2024

概括

我们开发了一种低成本的小型化系统,使用化学发光来检测水中的腺三酸盐 (ATP). 这种敏感的设备可以快速实时测量到0.2nM的ATP度.

科学领域:

分析化学分析化学
生物化学生物化学
环境科学环境科学

背景情况:

腺三酸盐 (ATP) 是生物活性和水质评估的关键生物标志物.
现有的ATP检测方法可能昂贵,复杂或缺乏实时功能.
对于广泛的环境监测,需要小型化,低成本的传感器.

研究的目的:

设计,制造和测试一种新的,具有成本效益的,小型化基于化学发光的腺三酸盐 (ATP) 检测系统.
为了实现高灵敏度和快速响应时间,在水样中实时测量ATP.

主要方法:

利用ATP和露西费林溶液之间的化学发光反应.
采用光倍增器 (SiPM) 进行高度敏感的实时光子检测.
制造了一个小型系统,以高效地处理和测量反应物.

主要成果:

该系统在水样中成功检测到腺三酸盐 (ATP).
达到了0.2 nM ATP的低检测极限.
证明了高灵敏度,测量结果为79.5 A/M.
呈现出快速反应时间,反应剂扩散和混合的特征为0.3 ± 0.1秒,相应的扩散速度约为44 ± 14mm2/s.

结论:

开发的低成本小型化系统为水中的ATP检测提供了灵敏和快速的方法.
这项技术在环境监测,水质评估和生物研究方面具有潜在的应用.
该系统的性能指标表明其适合实时现场测量.

关键词:

通过ATP生物发光检测检测.细菌充电检测检测检测的细菌充电检测扩散时间是扩散时间.微型传感系统是一个微型传感系统.光倍增器是一种光倍增器.

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