用聚合物纳米颗粒改善柏柏林在内通道灌中的抗菌功效
Célia Marques1,2, Liliana Grenho3, Maria Helena Fernandes3
1IUCS-CESPU, University Institute of Health Sciences (IUCS), Advanced Polytechnic and University Cooperative (CESPU), CRL, 4585-116 Gandra, Portugal.
Pharmaceutics
|June 27, 2024
概括
这项研究开发了柏林载荷的PLGA纳米颗粒用于内牙治疗. 这些纳米粒子有效地减少了浮游生物和生物膜细菌和酵母,可忽略的细胞毒性,显示出对牙感染的希望.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 微生物学 微生物学
背景情况:
- 根管中的生物膜对内牙治疗构成重大挑战.
- 柏柏林 (BBR) 是一种天然类化合物,具有与牙科相关的抗微生物,抗炎和抗氧化特性.
- 纳米颗粒输送系统为增强抗微生物疗效提供了潜力.
研究的目的:
- 为了合成和表征带有柏柏林 (BBR) 的聚 (乳酸-同-甘油酸) (PLGA) 纳米粒子.
- 为了评估BBR装载PLGA纳米颗粒对*Enterococcus faecalis*和*Candida albicans*的抗菌活性.
- 评估BBR载荷的PLGA纳米颗粒与牙纤维细胞的细胞相容性.
主要方法:
- 用纳米沉方法制备聚合物纳米颗粒.
- 确定了物理化学性质,包括尺寸,表面电荷和BBR封装效率 (77%).
- 对浮游生物和状细菌/酵母菌进行了抗菌活性和细胞毒性测试.
主要成果:
- 球形纳米粒子 (大约. 140nm) 带有稳定的表面电荷产生.
- 装有BBR的PLGA纳米粒子在1小时内显著减少了浮游生物*E. faecalis*和*C. albicans*在20μg/mL的水平.
- 在24小时后,在生物膜中观察到30-40μg/mL时,会座内牙细菌的活力下降.
- 对BBR载荷的纳米颗粒对口腔纤维细胞发现了微不足道的细胞毒性.
结论:
- 装有柏柏林的新型PLGA纳米粒子已成功合成和表征.
- 这些纳米颗粒在浮游生物和生物膜形式中表现出强大的抗微生物活性,对抗关键的内牙病原体.
- 开发的BBR装载纳米颗粒由于其有效性和安全性,代表了对内牙感染的有前途的补充治疗剂.
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