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在回收水中检测肠道病毒的体完整性检测

Pablo Puchades-Colera¹, Azahara Díaz-Reolid¹, Inés Girón-Guzmán¹

¹VISAFELab, Department of Preservation and Food Safety Technologies, Institute of Agrochemistry and Food Technology, IATA-CSIC, Av. Agustín Escardino 7, 46980 Valencia, Spain.

|June 27, 2024

概括

用于灌的废水再利用往往无法去除肠道病毒,这对公众健康构成风险. 大多数分析的回收水样本不符合欧盟标准,表明需要改进处理以确保水安全.

科学领域:

环境微生物学环境微生物学
水质工程水质工程
病毒学病毒学

背景情况:

气候变化和干旱迫使废水重新用于农业.
欧盟法规规定,灌用再生水中减少肠道病毒.
目前的废水处理可能无法充分使所有病毒病原体无活化.

研究的目的:

评估废水中人类肠道病毒 (诺病毒,轮状病毒) 和crAssphage的流行情况.
评价体内胆固醇作为病毒失活的可培养指标.
优化一种检测再生水中的传染性病毒颗粒的方法.

主要方法:

(RT) -qPCR用于检测人类诺罗病毒基因组I和II,轮状病毒和crAssphage.
对可培养的病毒指标进行体内胆细胞分析.
优化PMAxx-RT-qPCR以评估病毒囊完整性和传染性.

主要成果:

在60%的回收水中发现了体内体,这表明不完全无活化.
66%的样本使用PMAxx-RT-qPCR检测出肠道病毒呈阳性.
检测到的病毒度范围从2.79到7.30Log10基因组拷贝/L.

结论:

大多数分析的回收水样本不符合欧盟的水再利用标准.
在大量回收水中检测到有感染性的病毒颗粒.
目前的废水处理过程需要加强,以有效地去除病毒病原体.

关键词:

囊体完整性 (RT) -qPCRR的研究结果肠道病毒肠道病毒回收的水是回收的水.病毒污染指标病毒污染指标废水是废水,废水就是废水.

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