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Updated: Jun 8, 2026

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

Published on: May 2, 2011

一种鼻腔疫苗候选剂,含有SARS-CoV-2的三个抗原区域,以诱导更广泛的反应

Yadira Lobaina^1,2, Rong Chen^1,3, Edith Suzarte⁴

¹Research Department, China-Cuba Biotechnology Joint Innovation Center (CCBJIC), Lengshuitan District, Yongzhou 425000, China.

|June 27, 2024

概括

一种与CpG ODN-39M结合的新型嵌合蛋白 (S2NDH) 在小鼠中显示出强烈的免疫性,诱导对SARS-CoV-2和SARS-CoV-1核体蛋白质的交叉反应免疫力. 这种配方,添加了RBD,显示出作为宽频增强疫苗的希望.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
疫苗开发疫苗开发

背景情况:

感染SARS-CoV-2引发了针对病毒蛋白的免疫反应.
开发有效的抗萨尔贝科病毒疫苗需要引发广泛和持久免疫力的策略.

研究的目的:

创建和评估一种来自SARS-CoV-2核体 (N) 和尖端 (S2) 蛋白质的新型化学蛋白质 (S2NDH).
评估与CpG ODN-39M和/或RBD配制的S2NDH的免疫性和有效性,作为针对萨尔贝科病毒的鼻内疫苗候选剂.

主要方法:

在大肠杆菌中,一种仿制蛋白N-S2蛋白 (S2NDH) 的重组表达.
用CpG ODN-39M进行S2NDH粒子形成和聚合的表征.
通过S2NDH + ODN-39M和S2NDH + ODN-39M + RBD配方通过鼻腔免疫Balb/c小鼠.
评估幽默和细胞介导免疫 (CMI),包括对SARS-CoV-1的交叉反应,以及中和抗体 (NAb) 诱导.

主要成果:

S2NDH形成了10纳米粒子,并与COVID-19患者血清识别的CpG ODN-39M聚集在一起.
鼻内S2NDH + ODN-39M诱导了强大的抗N幽默免疫和对SARS-CoV-1 N蛋白的交叉反应性CMI,具有Th1模式.
单独S2NDH并没有诱导NAb或S2特异性CMI;然而,与RBD联合使用显著增强了对SARS-CoV-2和SARS-CoV-1的NAb诱导.
这种S2NDH + ODN-39M + RBD配方表现出明显的辅助作用,增强了RBD诱导的NAb.

结论:

该S2NDH + ODN-39M配方具有高度免疫性,诱导交叉反应性抗N免疫力.
将S2NDH + ODN-39M与RBD结合起来,可以产生一种强大的鼻内疫苗候选物,能够诱导对多种萨尔贝科病毒的中和抗体.
双价S2NDH + ODN-39M + RBD配方代表了广泛的萨尔贝科病毒增强疫苗的有希望的战略.

关键词:

在RBD中,RBD是RBD.S2 纤维纤维的使用情况这就是SARS-CoV-2病毒.基米蛋白质是基米蛋白质的组成部分.内接种疫苗内接种疫苗它是核体的核体.

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