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Updated: Jun 22, 2025

Bimolecular Fluorescence Complementation
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Bimolecular Fluorescence Complementation

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使用结合双光补充/GFP纳米体技术对蛋白相互作用的功能分析

Tetsuaki Miyake1,2,3, John C McDermott1,2,3

  • 1Department of Biology, York University, Toronto, ON, M3J 1P3, Canada.

Nucleic acids research
|June 27, 2024
PubMed
概括

这项研究引入了一种新的BiFC/GBP-nanobody方法,用于设计,可视化和分析转录因子 (TF) 模和它们与HDAC4.4等共同调节者的相互作用.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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