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每日暴露于低度的甲基甲酸甲会干扰HepG2细胞中的甲状腺激素通路

Taicheng An^1,2, Lirong Lu¹, Guiying Li^1,2

¹Guangdong-Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health, Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, China.

Fundamental research

|June 27, 2024

概括

每天接触到阻燃剂博二甲 (TBBPA) 会破坏甲状腺激素通路. 这种干扰会影响内分泌系统和Ras信号,对人类健康构成风险.

科学领域:

环境毒理学环境毒理学
内分泌学在内分泌学.
分子生物学分子生物学

背景情况:

甲 (TBBPA) 是一种广泛使用的阻燃剂,已知对人类健康和环境有不良影响.
阻燃剂可以干扰关键的生物通路,包括内分泌系统的生物通路.

研究的目的:

为了研究慢性低剂量TBBPA暴露对HepG2细胞基因调节的影响.
阐明TBBPA对甲状腺激素和副甲状腺激素信号通路的特定影响.
为了确定TBBPA暴露,甲状腺激素通路和Ras信号通路之间的关系.

主要方法:

暴露HepG2细胞在多代 TBBPA 的不同度.
定量聚合酶连锁反应 (qPCR) 用于分析基因表达水平.
与TBBPA和甲状腺激素受体对手的同时暴露实验.

主要成果:

长时间暴露于TBBPA导致甲状腺激素和副甲状腺激素信号通路的逐渐激活.
与甲状腺激素通路相关的基因表达在五代后显著上调 (1.15-8.54倍).
TBBPA抑制了Ras通路的激活,并降低了Ras基因表达的调节.
与甲状腺激素受体对手的同时暴露降低了相关受体的表达,证实了该途径的参与.

结论:

甲状腺二A (TBBPA) 干扰甲状腺激素信号通路.
由于TBBPA破坏了甲状腺激素通路,随后影响了更广泛的内分泌系统.
TBBPA的毒性影响与它与甲状腺激素受体的相互作用以及它对Ras信号通路的影响有关.

关键词:

内分泌系统内分泌系统低度的暴露暴露低度的暴露拉斯信号通道是RAS信号通道.TBBPA TBBPA 这是一个很好的方法.甲状腺激素受体受体是什么

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