在Sertuin 5驱动的介质组件和基于actin的迁移在小鼠卵细胞介质化中的介质性转移
Cong Ma1,2,3, Xueke Zhang1,2,3, Yingying Zhang1
1Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, No.218 Jixi Road, Hefei, 230022, China.
Heliyon
|June 27, 2024
概括
赛尔图因5 (Sirt5) 对于成功成熟的小鼠卵细胞至关重要. 它的抑制会扰乱螺旋组装,染色体对齐和行为动力学,导致成熟失败和细胞分裂异常.
科学领域:
- 细胞生物学 细胞生物学
- 生殖生物学 生殖生物学
- 生物化学 生物化学
背景情况:
- 锡尔图因5 (Sirt5) 在细胞过程中起作用,但其在卵细胞成熟中的功能尚不清楚.
- 卵细胞成熟对女性生育至关重要,涉及复杂的细胞事件,如介质分裂和极体挤出.
研究的目的:
- 为了研究Sirtuin 5 (Sirt5) 在小鼠卵细胞成熟中的作用.
- 阐明Sirt5影响卵细胞介质分裂和细胞周期进展的分子机制.
主要方法:
- 在小鼠卵细胞化过程中观察Sirt5表达的分析.
- 在卵细胞中实验性抑制Sirt5活性.
- 评估极体挤出,细胞循环进展,组装和染色体对齐.
- 分析了包括F-actin,cofilin和profilin1表达在内的actin动态.
主要成果:
- 在介质分裂期间,Sirt5表达是持续的,但在老化卵细胞中会下降.
- 抑制Sirt5导致成熟失败,细胞循环停止和异常的极体挤出.
- Sirt5的抑制导致了异常的线组装,染色体对齐失序,以及受损的线迁移.
- 抑制Sirt5破坏了actin动态,降低了酸化的cofilin/profilin1,并增加了细胞质F-actin.
结论:
- 赛尔图因5 (Sirt5) 对于小鼠卵子细胞的正常成熟至关重要.
- Sirt5调节了螺旋组织,染色体对齐和行为动态,这些对于成功的介质分裂和极体挤出至关重要.
- Sirt5的调节失调会影响卵细胞质量,并可能导致与年龄相关的生育能力下降.
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