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Updated: Jun 22, 2025

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快速和灵敏的细菌活性的量化,使用电比计光传感

Shengbin He1, Yajing Chen1, Jingtong Wang1

  • 1Key Laboratory of Longevity and Aging-related Diseases of Chinese Ministry of Education, Guangxi Colleges and Universities Key Laboratory of Biological Molecular Medicine Research, School of Basic Medical Sciences, Guangxi Medical University, Nanning, Guangxi 530021, P. R. China.

Analytical chemistry
|June 27, 2024
PubMed
概括

使用GelRed和SYTO 9的新型比度光方法可以有效地区分活菌和死菌. 这种敏感的技术通过准确评估细菌生存能力,有助于食品安全和药物开发.

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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