通过丰富转录因子结合动机,DNA转位子有利于de novo转录的出现
Marie Kristin Lebherz1, Bertrand Fouks2,3,4, Julian Schmidt1
1Institute for Evolution and Biodiversity, University of Münster, Münster, Germany.
Genome biology and evolution
|June 27, 2024
概括
可转移元素 (TE) 驱动非编码DNA的新转录的出现. TE插入引入了调节动机,促进了新的转录事件和基因起源.
科学领域:
- 基因组学就是基因组学.
- 进化生物学 进化生物学
- 分子生物学分子生物学
背景情况:
- 新基因 (de novo基因) 通过突变从以前非编码的基因组区域产生.
- 开放阅读框架 (ORF) 创建的机制是已知的,但对新转录的驱动因素的理解较少.
- 跨基因组存在一系列的转录水平,这表明了动态的调节过程.
研究的目的:
- 研究可转移元素 (TE) 在新转录的出现中的作用.
- 确定与新转录事件相关的调节机制,特别是转录因子动机.
- 使用多种多样的Drosophila melanogaster种群来检测和比较样本特定的de novo转录.
主要方法:
- 分析了来自七个杂交Drosophila melanogaster系的新组装的基因组和转录组.
- 将 de novo 转录与同类非转录区域和对照序列进行比较.
- 评估的重叠与可转换元素 (TEs) 和转录因子动机的丰富性在 de novo转录的上游.
主要成果:
- 新的转录显示了与可转换元素 (TE) 的显著重叠.
- 新的转录的出现与高的关氨酸-细胞因子含量和TE表达相关.
- de novo转录的上游区域富含转录因子动图,特别是在TE中,特别是DNATE.
结论:
- 可转移元件 (TE) 插入在启动de novo转录中起着至关重要的作用.
- 试验证书有助于促进新转录的出现的监管动机.
- 这种机制突显了TEs在基因组进化和基因新奇性中的重要性.
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