使用β-葡萄糖酶在深层欧性溶剂中对金氏化物化合物K的生物转化
Yinan Hong1, Yue Shi1, Yurou Fan1
1School of Environmental and Chemical Engineering, Xi'an Polytechnic University, No. 19 Jinhua South Road, Xi'an, 710048, Shaanxi, China.
Bioprocess and biosystems engineering
|June 27, 2024
概括
这项研究通过使用β-葡萄糖酶和绿色深溶解剂 (DES) 增强了金氏化物化合物K (CK) 的产生. 这种方法显著增加了CK的产量,使其在工业上可行.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 自然产品的合成自然产品的合成
- 绿色化学 绿色化学
背景情况:
- 金色化物化合物K (CK) 具有宝贵的药物特性,但难以分离.
- 目前的CK生产方法遭受低产量和复杂的分离过程.
- 酶转化提供了一种改善CK合成的潜在途径.
研究的目的:
- 为了优化使用β-葡萄糖酶将人参化物Rb1酶转化为CK.
- 调查使用深度浸泡溶剂 (DES) 来增强酶活性和产品产量.
- 通过在绿色溶剂系统中通过酶性水解来确定CK合成的最佳条件.
主要方法:
- 分子对接以预测酶基质相互作用.
- 单变体实验设计以优化DES组成和水解条件.
- 在DES介质中使用β-葡萄糖酶的酶性水解.
- 在DES中酶结构和构成的表征.
主要成果:
- 分子对接揭示了β-葡萄糖酶和人参酸之间的强烈相互作用.
- 在ChCl:EG (2:1,30%) 的最佳条件下,在pH值为5.0和50°C的DES产生了86.2%的CK.
- 与乙缓冲剂相比,DES系统提高了CK产量的1.24倍.
- 德斯没有对β-葡萄糖酶的结构或形状产生负面影响.
结论:
- 在DES中进行β-葡萄糖酶酶化水解是一种可行且高效的方法,用于合成罕见的金赛诺CK.
- 绿色DES溶剂系统适合于工业规模的CK生产.
- 这种方法为制药应用提供了一种可行且环保的替代方案.
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