在四个不同的试剂/仪器系统中,根据单一因素缺陷,确定APTT临界值
Hualan Huang1, Tianji Qu1, Min Hu1
1Department of Laboratory Medicine, The Second Xiangya Hospital, Central South University, Changsha, 410011, Hunan, China.
Annals of hematology
|June 27, 2024
概括
确定激活部分血栓形成时间 (APTT) 临界值需要对试剂进行特定评估. 这项研究确定了基于单个凝血因子缺陷的不同测试系统的新APTT临界值.
科学领域:
- 临床化学 临床化学
- 血液学 血液学 血液学
- 诊断 实验室科学 诊断 实验室科学
背景情况:
- 激活的部分血栓形成时间 (APTT) 临界值在不同研究中显著不同,缺乏针对特定检测系统的标准化.
- 国际血液学标准化委员会 (ICSH) 建议根据试剂类型,凝血因子敏感性和肝素反应,确定APTT临界值.
研究的目的:
- 为了确定试剂特定的APTT临界值.
- 根据单个凝血因子缺陷 (FVIII,FIX,FXI) 评估这些值.
主要方法:
- 在不同度的四个测量系统中,在因子缺乏血中测量了APTT值.
- 分析了来自孤立FVIII,FIX或FXI缺乏症患者的回顾性数据.
- 接收器操作特征 (ROC) 曲线评估了因子活性<5 IU/dL的诊断准确性.
主要成果:
- 在四种测试系统中观察到APTT值的显著差异 (P < 0.001).
- 试剂特定的关键APTT值被提出为:40.0秒 (Sysmex CS5100/Actin FSL),58.0秒 (Sysmex CS5100/Actin),51.8秒 (STA-R Evolution/STA-PTTA) 和64.8秒 (ACL TOP 700/HemosIL SynthasIL). 试剂的时间和时间是不同的.
- 对STA-PTTA的最佳ROC衍生值为55.8秒 (FVIII),54.3秒 (FIX) 和71.7秒 (FXI),显示出高灵敏度和特异性.
结论:
- 必须为特定的试剂确定APTT临界值.
- 为了准确的诊断解释,这些值应根据单个凝血因子缺陷的存在来确定.
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