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影响冷EM形成的薄膜中的宏分子方向的因素

Swati Yadav1, Kutti R Vinothkumar1

  • 1National Centre for Biological Sciences, Tata Institute of Fundamental Research, GKVK Post, Bellary Road, Bengaluru 560 065, India.

Acta crystallographica. Section D, Structural biology
|June 27, 2024
PubMed
概括

低温电子显微镜 (cryo-EM) 需要玻璃化样本. 这项研究揭示了导致偏好的宏分子方向的因素,为提高高分辨率成像的样本稳定性和数据质量提供了一个框架.

关键词:
低温电磁冷却仪的使用最喜欢的宏分子导向.表面活性剂是什么 表面活性剂是什么温度的温度的温度的温度的温度.薄膜是一种薄膜.

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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