Prx1/PHB2轴介导口腔白血病细胞衰老中的线粒细胞衰变
Yunping Lu1, Lingyu Li2, Jing Li2
1Department of Prosthodontics, Beijing Stomatology Hospital & School of Stomatology, Capital Medical University, Beijing 100050, China.
Pathology, research and practice
|June 27, 2024
概括
口腔白血病 (OLK) 的进展涉及Peroxiredoxin1 (Prx1) 与Prohibitin2 (PHB2) 结合,调节线粒细胞衰变和细胞衰老. Prx1Cys52作为氧化还原传感器,为这种口腔潜在恶性疾病提供了潜在的临床标.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 分子医学是分子医学.
背景情况:
- 口腔白血病 (OLK) 是最常见的口腔潜在恶性疾病 (OPMD),具有恶性转化潜力到口腔状细胞癌 (OSCC).
- 百氧化1 (Prx1) 和禁药2 (PHB2) 之间的相互作用与OLK进展有关,但其在线介导衰老中的作用尚不清楚.
研究的目的:
- 为了阐明 Prx1/PHB2轴在OLK老化中的机制.
- 调查Prx1Cys52在这个过程中作为氧化还原传感器的作用.
主要方法:
- 在人类正常,OLK和OSCC组织中对Ki67,p21,p16,PHB2和LC3进行免疫组合化学染色.
- 用H2O2.2治疗的Prx1 knockdown和Prx1C52S突变异位性口腔角质细胞 (DOK) 细胞中线粒细胞和线粒体功能的分析.
- 使用现场近距离结合试验和共免疫沉检测Prx1-PHB2相互作用.
主要成果:
- 基67阳性率逐渐从正常增加到OLK和OSCC组织.
- 与正常和OSCC组织相比,OLK中观察到高的p21,p16,PHB2和LC3表达.
- 在H2O2诱导的DOK细胞中,Prx1/PHB2轴调节了线粒和衰老;Prx1C52S突变损害了Prx1-PHB2相互作用,导致ROS增加和细胞循环停止.
结论:
- Prx1Cys52作为氧化还原传感器,与PHB2结合,以调节OLK细胞衰老中的线粒细胞衰变.
- Prx1/PHB2轴在OLK病变发生过程中起着至关重要的作用.
- Prx1Cys52为管理OLK提供了潜在的临床点.
相关概念视频
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
The Intrinsic Apoptotic Pathway
6.5K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.5K
Phagocytosis of Apoptotic Cells
3.8K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...
Normal cells contain receptors that prevent them from being recognized...
3.8K
Replicative Cell Senescence
3.6K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
3.6K
Restarting Stalled Replication Forks
5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K
Delivery Pathways to the Lysosome
6.2K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.2K


