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热冲击蛋白DNAJB8通过自抑制伪狂犬病病毒的复制

Xiaoyong Chen¹, Wenfeng Li²

¹Xingzhi College, Zhejiang Normal University, Jinhua, Zhejiang, PR China.

Veterinary microbiology

|June 27, 2024

概括

热冲击蛋白DNAJB8通过促进自性来负面调节伪病毒 (PRV) 复制. 转录因子SOX30影响DNAJB8的表达,影响病毒病原和宿主病毒相互作用.

科学领域:

病毒学病毒学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

伪狂热病毒 (PRV) 感染依赖于宿主因素.
了解新型宿主病毒相互作用对于PRV病变研究至关重要.

研究的目的:

识别和描述调节PRV复制的宿主因素.
阐明DNAJB8和SOX30在PRV感染中的作用.

主要方法:

定量PCR和西式斑点测试以评估DNAJB8的表达.
基因过度表达和淘汰,以评估DNAJB8对PRV复制的影响.
自试验是为了研究它的作用机制.
对SOX30在DNA上的调节作用的分析JB8.8.

主要成果:

在PRV感染期间,DNAJB8表达 (mRNA和蛋白质) 的下调.
DNAJB8的过度表达抑制了PRV的复制;DNAJB8的淘汰增强了它.
DNAJB8促进细胞自,从而抑制PRV复制.
转录因子SOX30调节DNAJB8的表达,影响病毒复制.

结论:

DNAJB8作为PRV复制的负调节剂.
SOX30通过调节DNAJB8. 的调节来调节PRV复制.
这些发现扩大了对DNAJB8和SOX30在病毒复制和病毒与宿主相互作用中的理解.

关键词:

自自是一种自的过程.在DNAJB8中使用.伪狂犬病病毒病毒.在SOX30中,SOX30是SOX30的.

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