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开发基于P30蛋白的间接ELISA检测非洲猪瘟抗体,使用HEK293F表达系统检测

Huahan Chen¹, Junhai Zhu¹, Xuefeng Niu²

¹State Key Laboratory for Animal Disease Control and Prevention, South China Agricultural University, Guangzhou 510642, China; African Swine Fever Regional Laboratory of China (Guangzhou), Guangzhou 510642, China; Key Laboratory of Zoonoses, Ministry of Agriculture and Rural Affairs, Guangzhou 510642, China; National and Regional Joint Engineering Laboratory for Medicament of Zoonoses Prevention and Control, Guangzhou 510642, China.

Veterinary journal (London, England : 1997)

|June 27, 2024

概括

这项研究开发了一种敏感的间接ELISA,使用ASFV p30蛋白检测早期抗体,改善了对猪的非洲猪瘟诊断. 这种方法为有效的疾病监测提供了高特异性和可重复性.

科学领域:

兽医病毒学兽医病毒学
免疫诊断免疫诊断免疫诊断免疫诊断

背景情况:

非洲猪瘟 (ASF) 是一种高度致命的猪病,由非洲猪瘟病毒 (ASFV) 引起.
由于缺乏疫苗和抗病毒药物,需要强大的诊断工具来控制ASF.
低毒性ASFV菌株的出现使早期检测变得复杂,导致错误阳性.

研究的目的:

开发一种敏感和特定的间接ELISA用于对ASFV的抗体的早期检测.
利用p30蛋白,一个早期表达的抗原,以提高诊断准确度.

主要方法:

使用HEK293F悬浮细胞表达系统生产复合p30蛋白.
建立和验证使用p30蛋白作为涂层抗原的间接ELISA.
测试灵敏度,特异性和可重复性的评估,包括与商用试剂盒进行比较.

主要成果:

间接ELISA显示出高灵敏度,标位为1:12800.
该试验表现出极好的特异性和可重复性.
与商业诊断套件相比,观察到高达98.148%的巧合率.

结论:

成功开发了一种敏感的间接ELISA用于ASFV抗体检测.
这种利用p30蛋白的方法是用于在猪群中早期和准确诊断ASF的宝贵工具.
开发的测试有助于有效监测和控制ASFV疫情.

关键词:

非洲猪瘟是非洲猪瘟的一种疾病.HEK293F表达系统表达方式间接的ELISA是一个间接的ELISA.P30 再组合蛋白质的蛋白质.

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Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
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