相关实验视频

Updated: Jun 22, 2025

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

在高CO2条件下调节大豆结节的GmHSP23.9的作用

Bingjie Niu¹, Nan Bai¹, Xiaofeng Liu¹

¹College of Agriculture, Shanxi Agricultural University, Taigu 030801, China.

International journal of biological macromolecules

|June 27, 2024

概括

升高的CO2通过调节GmHSP23.9,一个小热冲击蛋白,增加大豆结节数量. 这种基因对结节信号通路至关重要,其敲除可以防止CO2诱导的结节增加.

科学领域:

植物分子生物学植物分子生物学
农作物科学农作物科学
生物化学生物化学

背景情况:

豆类-树菌共生可以提高作物产量.
已知高度的二氧化碳会增加大豆结节的数量.
在二氧化碳中介大豆结节的基础上存在的分子机制尚不清楚.

研究的目的:

在CO2升高的情况下,确定调节大豆结节的基因.
研究一个特定的小热冲击蛋白 (sHSP),GmHSP23.9,在这个过程中的作用.
阐明由GmHSP23.9.9调制的信号通路.

主要方法:

转录组分析以确定差异表达基因 (DEG).
转基因大豆植物的生成 (过度表达,RNA干扰,CRISPR-Cas9) 用于GmHSP23.9.9.
对结节数量,根毛变形和信号通路中的基因表达的分析.

主要成果:

GmHSP23.9,一个sHSP,被确定在高CO2和根茎菌感染下显著上调.
调节GmHSP23.9表达改变了结节数和根毛发的发育.
GmHSP23.9影响Nod因子和AON信号通路.
淘汰GmHSP23.9取消了CO2升高下的结节数量的增加2.

结论:

GmHSP23.9是大豆结节的关键积极调节者,在高CO2的条件下.
这项研究揭示了sHSPs在豆类共生和二氧化碳反应中的新角色.
了解GmHSP23.9为优化豆类作物产量提供了洞察力.

关键词:

升高的二氧化碳 (CO) 度小热冲击蛋白 (sHSPs) 是一种热冲击蛋白.豆豆结节在豆豆结节中对转录组进行分析.

更多相关视频

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Published on: December 23, 2017

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

相关概念视频

Key Elements for Plant Nutrition

Key Elements for Plant Nutrition

Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...

Regulation of Transpiration by Stomata

Regulation of Transpiration by Stomata

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.

Plant Hormones

Plant Hormones

Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.