探索Trichoderma reesei作为生产红醇的替代宿主
Audrey Masi1,2, Georg Stark1, Johanna Pfnier1
1Christian Doppler Laboratory for Optimized Expression of Carbohydrate-Active Enzymes, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorfer Str. 1a, 1060, Vienna, Austria.
Biotechnology for biofuels and bioproducts
|June 27, 2024
概括
三虫可以从葡萄糖中产生红醇,但需要优化,以节省成本,大规模生产这种低热量甜味剂. 对聚醇代谢的进一步研究可能会开启新的应用.
科学领域:
- 生物技术是生物技术.
- 微生物发酵 微生物发酵
- 代谢工程是代谢工程.
背景情况:
- 红醇是一种天然的多和低热量甜味剂,由于高昂的成本与富含葡萄糖基质相关,因此面临生产限制.
- 探索像Trichoderma reesei这样的替代微生物宿主,可以利用纤维素生物质,为更可持续的红醇生产提供了一条道路.
研究的目的:
- 评估Trichoderma reesei作为红醇生物合成的宿主.
- 为了确定最佳的碳和来源,以及用于生产红的关键环境参数 (pH,温度).
- 评估使用T. reesei.扩大红醇生产的可行性.
主要方法:
- 培养 Trichoderma reesei 在各种碳和来源.
- 优化发酵参数,包括pH值和温度.
- 振动瓶实验,随后进行生物反应器试验,以评估可扩展性.
主要成果:
- 特里科德尔玛reesei合成的红醇完全来自葡萄糖,而不是西洛斯或乳糖.
- 尿素和酵母提取物被证明是比或酸盐更有效的源.
- 红醇的产生受到pH值和温度的显著影响,在生物反应器扩展过程中遇到了挑战.
结论:
- 特里科德尔马·里西 (Trichoderma reesei) 显示出红醇生产的潜力,达到1g/L,但需要进一步优化以提高生产率.
- 这项研究提供了关于T. reesei的多代谢的见解,这与糖醇和曼尼托尔生产有关.
- 建议T. reesei的红醇生产,糖解和酸途径之间存在潜在的联系.
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