在线呼吸分析的内部标准加法系统
Cedric Wüthrich1, Timon Käser1, Renato Zenobi1
1Department of Chemistry and Applied Biosciences, ETHZ, Zurich, CH 8093, Switzerland.
Analytical chemistry
|June 27, 2024
概括
一个新的系统可以使用二次电喷射电离-质谱法 (SESI-MS) 进行定量呼吸分析. 这种方法通过引入可控气体标准以进行准确的测量来改善呼吸代谢.
科学领域:
- 分析化学 分析化学
- 代谢学 代谢学 代谢学
- 生物技术是生物技术.
背景情况:
- 使用二次电喷射电离与质谱学 (SESI-MS) 结合的呼吸分析是代谢学的一个敏感技术.
- 由于变化和潜在的污染物,呼吸代谢的定量评估具有挑战性.
研究的目的:
- 开发一种用于对呼吸样本进行控制引入气体的系统,用于定量SESI-MS分析.
- 为了使标准的加法实验能够准确量化呼吸中的代谢物.
主要方法:
- 设计了一个系统,其中包括用于控制气体标准生成,加湿,呼吸稀释和标准注射的质流控制器.
- 实时标准加法实验是使用气息样本中的氨酸和黄油酸进行的.
主要成果:
- 开发的系统成功地将可控量的气体引入到呼吸样本中.
- 通过成功实时标准添加化和黄油酸来证明了定量能力.
- 该系统的呼吸稀释能力有助于过出污染化合物.
结论:
- 新系统提高了通过SESI-MS获得的呼吸代谢学数据的质量和稳定性.
- 这一进步有助于更可靠地对呼吸中的挥发性有机化合物的定量分析.
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