相关实验视频

Updated: Jun 22, 2025

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

13.2K

PROMER技术:一种新的实时PCR工具,可实现高特异性和高灵敏性的点突变的多重检测

Hwanhee Nam1, Esder Lee2, Hichang Yang2

  • 1Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, United States.

Biology methods & protocols
|June 28, 2024
PubMed
概括

我们开发了PROMER技术,这是一种新的实时PCR方法,用于精确检测循环瘤DNA (ctDNA) 中罕见的癌症突变. 这一进步改善了用于癌症管理的液体活检分析.

关键词:
在KRAS突变中发生突变.没有细胞的DNA DNA.在循环中的瘤DNA.液体活检活检液体活检在实时PCR中进行实时PCR.

更多相关视频

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

8.6K
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

11.7K

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.1K