干涉度光显微镜与纳米粒子跟踪分析的比较,用于研究细胞外囊泡和细菌菌体
Romain Sausset1,2,3, Zuzana Krupova4, Eric Guédon5
1Micalis Institute, INRAE, AgroParisTech Université Paris-Saclay Jouy-en-Josas France.
Journal of extracellular biology
|June 28, 2024
概括
一种新的干扰度光显微镜 (ILM) 装置Videodrop显示出对量化细胞外囊泡 (EV) 和细菌菌体 (菌体) 的前景. 虽然对菌体的敏感性较低,但它为更大的EV提供了节省时间的度分析.
科学领域:
- 纳米技术纳米技术
- 显微镜的使用方法
- 生物技术是生物技术.
背景情况:
- 细胞外囊泡 (EV) 和菌体 (菌体) 是重要的生物纳米粒子,在医学,生物学和生态系统中发挥着不断扩大的作用.
- 这些纳米粒子的精确量化和尺寸确定对于研究至关重要.
- 需要新的工具来满足对分析EV和菌体的日益增长的需求.
研究的目的:
- 为了比较一种新的干扰度光显微镜 (ILM) 装置Videodrop的性能,与两个纳米粒子跟踪分析 (NTA) 装置 (NanoSight和ZetaView) 相比.
- 评估这些设备对于分析细胞外囊泡 (EVs) 和菌体 (菌体) 的适用性.
主要方法:
- 使用Videodrop,NanoSight和ZetaView分析来自各种来源 (细菌,便,牛奶,人体细胞) 和菌体 (30-120纳米直径) 的EV.
- 在设备之间比较灵敏度,计数精度,尺寸分布精度和时间效率.
- 对纳米粒子检测极限和度确定能力的评估.
主要成果:
- 纳米粒子跟踪分析 (NTA) 仪器准确地列出了大多数菌体,而视频滴只检测到较大的菌体,这表明敏感度值较低.
- 视频滴表现出与NTA设备可比的性能,用于确定真核生物EV样本的度.
- 纳米Sight提供了最精确的尺寸分布,但Videodrop的时间效率要高得多.
结论:
- 视频滴是一种节省时间的设备,用于分析大于90nm的纳米粒子,特别是用于真核细胞细胞外囊泡 (EV) 度测量.
- 虽然NTA设备对菌体等较小的纳米颗粒具有更高的灵敏度,但Videodrop为特定应用提供了有价值的替代方案.
- 视频滴值得考虑涉及更大的纳米粒子的高通量研究,以补充现有的NTA技术.
更多相关视频
09:16Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
Published on: January 7, 2019
9.8K
09:19Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
Published on: March 28, 2021
8.4K
相关概念视频
Overview of Electron Microscopy
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Overview of Microscopy Techniques
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Two-Dimensional Microscopy in Microbiology
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Three-Dimensional Microscopy in Microbiology
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Methods to Assess Microbial Populations
Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...
