Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

What are Lipids?01:38

What are Lipids?

197.4K
Overview
197.4K
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

7.2K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
7.2K
Membrane Fluidity01:26

Membrane Fluidity

11.1K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
11.1K
Structure of Lipids03:38

Structure of Lipids

87.8K
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
87.8K
Blood Studies for Cardiovascular System III: Serum Lipid Profile01:25

Blood Studies for Cardiovascular System III: Serum Lipid Profile

154
Understanding serum lipids is crucial for maintaining cardiovascular health and preventing heart disease and stroke.
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
154
Lipid-derived Compounds in the Human Body01:31

Lipid-derived Compounds in the Human Body

4.3K
Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various  biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin,...
4.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Molecular action of NZ2114, a superior plectasin derivative.

npj antimicrobials and resistance·2026
Same author

Dynamics of viral self-assembly, viral genome packaging, and virus-cell interactions studied by optical tweezers.

European biophysics journal : EBJ·2026
Same author

MDA5 generates compact ribonucleoprotein complexes via ATP-dependent double-stranded RNA unwinding.

Nucleic acids research·2026
Same author

Impact of modification of envelope proteins on the mechanical properties of HIV virus-like particles.

Journal of the mechanical behavior of biomedical materials·2026
Same author

Influence of Substrate on Supported Lipid Bilayers: Membrane Adhesion, Stretching, Pores, and Remodeling.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Standardization of cross-site biophysical studies of bovine insulin amyloids is challenged by structural polymorphism.

European biophysics journal : EBJ·2026

相关实验视频

Updated: Jun 22, 2025

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
11:13

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Published on: May 9, 2019

8.8K

脂蛋白颗粒具有独特的机械特性.

Melissa C Piontek1, Wouter H Roos1

  • 1Moleculaire Biofysica Zernike Instituut, Rijksuniversiteit Groningen Groningen The Netherlands.

Journal of extracellular biology
|June 28, 2024
PubMed
概括

这项研究使用原子力显微镜对脂蛋白 (LPs) 进行了表征,揭示了不同的机械性质. 这些发现有助于区分LP与细胞外囊泡,并了解LP在动脉样硬化中的作用.

科学领域:

  • 生物物理学的生物物理.
  • 生物化学 生化学
  • 心血管研究研究心血管研究

背景情况:

  • 脂蛋白 (LPs) 的大小与细胞外囊泡 (EVs) 类似,导致共同隔离的挑战.
  • 血LP,包括胆米克龙 (CM),VLDL,LDL和HDL,都与胆固醇运输和动脉样硬化有关.
  • 了解LP的机械特性对于将其与EV区分开来以及它们在疾病中的作用至关重要.

研究的目的:

  • 使用原子力显微镜 (AFM) 机械表征人体血脂蛋白.
  • 评估通常用于EVs的纳米沉积技术对LP分析的适用性.
  • 为了识别不同LP亚型 (CM,VLDL,LDL,HDL) 的独特机械特征.

主要方法:

  • 原子力显微镜 (AFM) 用于对脂蛋白的纳米沉积分析.
  • 测量了机械性能,包括曲模量和表面粘附行为.
  • 在不同类型的脂蛋白中进行了对比分析.

主要成果:

  • 低密度脂蛋白 (LDL) 显示出较高的曲模量,相比于基洛米克龙 (CM) 和非常低密度脂蛋白 (VLDL).
  • 在缩后,CM显示了粒子崩,而HDL显示了表面粘附后的高度非常低.
  • 在LP亚型之间观察到明显的机械差异,有助于它们的表征.
关键词:
原子力显微镜 (AFM) 的使用基隆微粒 (CM) 是一种微粒.高密度脂蛋白 (HDL) 是一种高密度脂蛋白.脂蛋白 (LPs) 是一种蛋白质.低密度脂蛋白 (LDL) 是一种低密度脂蛋白.机械性能 机械性能 机械性能非常低密度脂蛋白 (VLDL) 是一种非常低密度脂蛋白.

更多相关视频

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
09:37

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Published on: August 15, 2014

43.6K
Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration
09:29

Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration

Published on: January 19, 2020

8.4K

相关实验视频

Last Updated: Jun 22, 2025

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
11:13

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Published on: May 9, 2019

8.8K
Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
09:37

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Published on: August 15, 2014

43.6K
Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration
09:29

Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration

Published on: January 19, 2020

8.4K

结论:

  • AFM纳米印记是一种可行的方法来表征脂蛋白的机械性质.
  • 机械差异可以帮助区分脂蛋白亚型和细胞外囊泡.
  • 这些见解促进了对LP行为及其在心血管健康和疾病中的作用的理解.