Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

3.4K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.4K
Viral Structure00:56

Viral Structure

62.1K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
62.1K
Overview of Exosomes01:36

Overview of Exosomes

2.7K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.7K
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

2.5K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
2.5K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

8.5K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.5K
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

6.1K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
6.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Recent Progress on Carbon-Dots-Based Probes for Microbial Labeling and Versatile Analysis Applications.

Biosensors·2026
Same author

Lanthanide-doped luminescent lateral flow immunoassay for the ultrasensitive and quick detection of methyltestosterone in fish samples.

Analytical methods : advancing methods and applications·2026
Same author

Molecularly Encoded Regulation of DNA Self-Assembly Crystallization in a Closed Homogeneous Solution System.

Nano letters·2026
Same author

Ultrabright Carbon Dots as an Emerging Fluorescent Probe for Multivalent Labeling and Highly Sensitive Detection of Extracellular Vesicles.

ACS applied materials & interfaces·2026
Same author

Advanced Encapsulation Technologies for Extracellular Vesicles: From Single Units to Macroscale Packaging.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Recent Advances on Rapid Detection Methods of Steroid Hormones in Animal Origin Foods.

Biosensors·2025

相关实验视频

Updated: Jun 22, 2025

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions
09:36

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Published on: August 26, 2021

3.8K

细胞外囊泡球形核酸细胞外囊泡球形核酸

Hao Chen1,2, Qiaojiao Ding3, Lin Li1

  • 1Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Ningbo Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315300, China.

JACS Au
|June 28, 2024
PubMed
概括
此摘要是机器生成的。

研究人员开发了细胞外囊球状核酸 (EV-SNA) 纳米结构,以增强药物输送. 这些新型EV-SNA提供了更好的生物相容性和细胞传递,为先进的纳米医学应用铺平了道路.

更多相关视频

Extraction of Extracellular Vesicles from Whole Tissue
09:03

Extraction of Extracellular Vesicles from Whole Tissue

Published on: February 7, 2019

15.0K
Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
12:27

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

4.7K

相关实验视频

Last Updated: Jun 22, 2025

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions
09:36

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Published on: August 26, 2021

3.8K
Extraction of Extracellular Vesicles from Whole Tissue
09:03

Extraction of Extracellular Vesicles from Whole Tissue

Published on: February 7, 2019

15.0K
Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
12:27

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

4.7K

科学领域:

  • 生物材料科学 生物材料科学
  • 纳米技术 纳米技术
  • 分子生物学分子生物学

背景情况:

  • 细胞外囊泡 (EVs) 是细胞分泌的天然纳米粒子,具有治疗潜力.
  • 目前对EV操纵和输送的局限性阻碍了它们的临床应用.
  • 需要强大的和可编程的纳米结构来实现高效的基于电动汽车的疗法.

研究的目的:

  • 开发一种新的纳米结构,细胞外囊球体核酸 (EV-SNA),用于增强细胞传递.
  • 设计可编程和可扩展的基于电动汽车的纳米载体.
  • 提高核酸治疗药物的传递效率和生物相容性.

主要方法:

  • 通过天然EVs与胆固醇修饰的寡核酸一起进行水性联合组装来构建EV-SNA.
  • 使用可编程的核酸外,响应AND逻辑门,用于囊泡组合控制.
  • 与人工脂肪体SNA相比,评估了EV-SNA稳定性,细胞递送增强和生物相容性.

主要成果:

  • 在室温下,EV-SNA在1个月内表现出稳定性.
  • 通过使用各种电动汽车来源,实现了10-20倍的蜂传输能力提升.
  • 在初级干细胞中,EV-SNA显示出优越的生物相容性和更有效的反感性寡核酸的传递.
  • 证明了EV-SNA在提供功能性EV用于免疫调节方面的能力.

结论:

  • EV-SNA代表了一个可扩展,可编程和高效的纳米结构,用于基于电动汽车的应用.
  • 这种新的平台增强了核酸治疗药物的传递,特别是在具有挑战性的细胞类型中.
  • 在推进药物输送,纳米疫苗和再生医学方面,EV-SNA具有显著的前景.