使用表面增强空间偏移光谱 (SESORS) 的体内成像:平衡采样频率以改善整体图像采集
Fay Nicolson1,2, Bohdan Andreiuk2,3, Eunah Lee4
1Department of Cancer Biology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA 02215, USA.
Npj imaging
|June 28, 2024
概括
表面增强的空间偏移共振拉曼光谱 (SESORRS) 能够更快,更深的体内瘤成像. 这种新的技术可以提高信号噪声比率,以更少的测量来检测深层质母细胞瘤及其边缘.
科学领域:
- 光学成像技术的成像
- 生物医学光谱学 生物医学光谱学
- 纳米技术纳米技术
背景情况:
- 在体内以高选择性和特异性对深层瘤进行成像是光学成像的一个重大挑战.
- 表面增强共振拉曼散射 (SERRS) 纳米粒子提供有针对性的对比度,但需要时间密集的光谱采集.
- 空间偏移拉曼光谱 (SORS) 提供深度分辨率信息,但速度和分辨率可能受到限制.
研究的目的:
- 开发和验证一种新技术,即表面增强空间偏移共振拉曼光谱 (SESORRS),用于深层瘤的快速,高特异性体内成像.
- 增强SORS系统的功能,以提高光收集效率和信号噪声比.
- 证明检测原发性瘤,划分边缘,识别二次病变的能力,并减少获得时间和测量.
主要方法:
- 结合了SERRS纳米粒子与修改后的SORS系统,称为SESORRS,结合了增加的数值光圈镜头和纤维,并延长了工作距离.
- 研究了四种不同的采样频率 (12,35,176,651个数据点) 用于SESORRS对小鼠GL261-Luc质母细胞瘤的成像.
- 量化了信号与噪声比率 (SNR) 的改进,并将采集时间与以前的SORS系统相比较.
主要成果:
- 与以前的系统相比,修改后的SORS仪表显示SNR有300%的改善.
- 通过SESORRS,可以从深部质母细胞瘤通过头骨获取独特的拉曼光谱,其功率密度低,整合时间显著缩短 (0.5秒).
- 该技术成功地绘制了瘤区域,划出了更高采样频率的边缘,并确定了更深的次要病变,与MRI和H&E染色一致.
结论:
- SESORRS显著改善了体内深层瘤的检测,提供了增强的SNR,光谱分辨率和深度获取能力.
- 这种方法可以更快地导航较大的组织区域,识别感兴趣的区域,并随后进行高分辨率成像.
- 通过完整的头骨,SESORRS证明了通过完整的头骨检测二级,更深层次的病变的潜力,为神经瘤学研究和诊断提供了宝贵的工具.
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