相关实验视频

Updated: Jun 22, 2025

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Published on: June 23, 2011

一种新型的多目标自组合-siRNA复合体,用于同时抑制SARS-CoV-2宿主细胞相互作用和复制

Martina Tuttolomondo¹, Stephanie Thuy Duong Pham¹, Mikkel Green Terp¹

¹Department of Molecular Medicine, Unit of Cancer and Inflammation Research, University of Southern Denmark, 5000 Odense, Denmark.

Molecular therapy. Nucleic acids

|June 28, 2024

概括

一种新型,HE25,提供小干扰RNA (siRNA) 抑制SARS-CoV-2复制. 这种-siRNA复合体显示出治疗COVID-19和未来病毒威胁的前景.

科学领域:

生物化学生物化学
病毒学病毒学
药物运输药物运输药物运输

背景情况:

严重的COVID-19需要超越疫苗的先进治疗方法.
小干扰RNA (siRNA) 提供了一种通过向病毒基因来抑制SARS-CoV-2复制的策略.
穿透细胞的可以增强siRNA的稳定性和细胞吸收,以便有针对性地传递.

研究的目的:

开发和评估针对针对SARS-CoV-2的siRNA的基于的输送系统.
调查HE25促进siRNA传递和病毒抑制的机制.
为了评估siRNA/HE25复合体对SARS-CoV-2变体的疗效in vitro和in vivo.

主要方法:

开发用于绑定SARS-CoV-2入口受体 (ACE2,整体,NRP1) 的HE25.
利用HE25作为一种载体,将依赖RNA的RNA聚合酶siRNA输送到细胞中.
通过histidine-proton海绵效应研究siRNA释放,以及由cathepsinB进行HE25裂变.
评估SARS-CoV-2 (祖先和Omicron BA.5) 复制的抑制在体外.
在静脉注射或吸入后评估HE25的生物分布in vivo.

主要成果:

HE25有效地将siRNA传递到细胞中,抑制了SARS-CoV-2的复制.
该siRNA/HE25复合体对祖先的SARS-CoV-2和Omicron BA.5变种都表现出有效性.
在体内给药时,HE25在肺组织中积累,表明呼吸道输送的潜力.
在用siRNA/HE25复合体治疗的肺细胞中观察到基因沉默.

结论:

这种HE25可以作为针对SARS-CoV-2的siRNA的有效传递载体.
开发的siRNA/HE25复合体在体外和体内表现出对SARS-CoV-2的强有力的抗病毒活性.
这种治疗策略具有管理COVID-19和打击未来病毒爆发的潜力.

关键词:

在ACE2中,ACE2是ACE2.在 COVID-19 疫情中,MT:寡核酸:治疗方法和应用.在RGD中,RGD是RGD.它们是RNAiRNAi.这是SARS-Cov-2病毒.卡瑟普辛B B的情况细胞透的.整合物是一种整合物.siRNA 是一个RNA.

更多相关视频

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

相关概念视频

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...