作为一种强大的酵母表达系统,Komagataella phaffii (Pichia pastoris) 作为一种强大的酵母表达系统,用于生物制品生产
1Department of Molecular Biology and Genetics, Faculty of Science, Atatürk University, 25240 Erzurum, Turkey.
Frontiers in bioscience (Elite edition)
|June 28, 2024
概括
生物技术酵母 Komagataeella phaffii (K. phaffii) 是一个强大的平台,用于生产用于制药和工业用途的重组蛋白质和生物分子. 优化策略确保有效生产有价值的产品,如酶和疫苗.
科学领域:
- 生物技术和制药科学 生物技术和制药科学
- 分子生物学和基因工程分子生物学和基因工程
- 生物工艺工程 生物工艺工程
背景情况:
- 科马加泰 (Komagataeella) phaffii (K. phaffii),也被称为Pichia pastoris,是一种在生物技术中广泛使用的甲基营养酵母.
- 由于分泌表达,高细胞密度和稳定的基因整合,它是重组蛋白质生产的高效平台.
- K. phaffii有着经过验证的记录,产生了5000多种重组蛋白质,制造了70多种商业产品.
研究的目的:
- 审查K. phaffii表达系统及其在工业和生物制药蛋白质生产中的意义.
- 讨论生物处理和基因改造战略,以提高高效的蛋白质生产.
- 突出K. phaffii作为细胞工厂的适应性,用于各种生物分子合成.
主要方法:
- 对K. phaffii应用和表达系统的现有文献的审查.
- 优化策略的分析,包括代码偏差,基因剂量,促进剂,信号和环境因素.
- 检查K. phaffii在生产酶,生物制药和亚单元疫苗中的作用.
主要成果:
- K. phaffii 能够产生重组蛋白质,可占细胞总蛋白质的30%或释放的蛋白质的80%.
- 使用K. phaffii的300多个工业过程已经获得许可,生产酶 (例如,西兰酶,脂酶) 和生物制药 (例如,胰岛素,HBsAg).
- K. phaffii是合成子单元疫苗和其他复杂生物分子的首选宿主.
结论:
- K. phaffii是一种高效和适应性的表达系统,对工业和制药应用至关重要.
- 多层次的优化是最大限度地提高重组蛋白产量的关键,条件是针对特定的蛋白量身定制的.
- K. phaffii将继续在研究和工业环境中成为一个强大的表达系统.
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