凝血杆菌通过Apostichopus japonicus中的酸盐-FFAR2-NF-κB-MLCK-MLC轴恢复病原体诱导的肠道功能障碍
Mingshan Song1, Shanshan Zhang1, Zhen Zhang1
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, China.
mSystems
|June 28, 2024
概括
海中的皮肤综合征 (SUS) 与肠道细菌失衡有关. 用Bacillus coagulans和酸盐恢复肠道微生物群有益于肠道健康,并预防疾病.
科学领域:
- 海洋生物学 海洋生物学
- 水产养殖是水产养殖的一种方式.
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
背景情况:
- 在 *Apostichopus japonicus* 中,皮肤综合征 (SUS) 导致高死亡率,由 *Vibrio splendidus* 驱动.
- SUS与肠道微生物群 (IM) 失生症,改变的短链脂肪酸 (SCFA) 和肠道屏障损伤有关.
- 在SUS期间IM在调解肠道功能障碍中的作用尚不清楚.
研究的目的:
- 通过肠道微生物种移植 (IMT) 调查IM和SUS发展之间的联系.
- 确定能够恢复肠道平衡的细菌及其机制.
- 探索SCFA产生细菌和SCFA在预防和控制SUS的治疗潜力.
主要方法:
- 在A. japonicus*中进行综合性肠道微生物群移植 (IMT).
- 隔离和识别产生SCFA的细菌,特别是凝血菌.
- 乙酸的使用和FFAR2受体分析,包括*in vivo*敲击实验.
- 对NF-κB-MLCK-MLC信号通路和肠道屏障完整性标记物的分析 (ZO-1,Occludin).
主要成果:
- 来自健康捐赠者的肠道微生物移植恢复了SUS接受者的IM和肠道屏障功能.
- *Bacillus coagulans*成功地恢复了IM社区和肠道屏障功能.
- 乙酸盐的使用通过与FFAR2结合,抑制NF-κB-MLCK-MLC通路,并上调ZO-1和Occludin表达,恢复了患病的A. japonicus*的肠道平衡.
结论:
- *凝血杆菌*和酸盐可以恢复患有SUS的*A. japonicus*的肠道屏障功能.
- 乙酸/FFAR2/NF-κB-MLCK-MLC轴对于调解恢复肠道平衡至关重要.
- 这些发现为从生态角度控制和预防SUS爆发提供了新的见解.
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