相关实验视频
Updated: Jun 22, 2025

11:42
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
Published on: April 7, 2017
9.4K
外体miR-196a-5p通过抑制ITM2B来促进食道状细胞癌的恶性进展
Min Huang1, Shuang Li1, Hai Zeng1
1Department of Oncology, First Affiliated Hospital of Yangtze University, Jingzhou, Hubei, China.
Pathology international
|June 28, 2024
概括
携带microRNA-196a-5p的外体促进食道状细胞癌 (ESCC) 的进展. 针对这种微RNA及其与ITM2B的相互作用,可能为ESCC治疗提供新的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 外基因组通过携带微RNAs (miRNAs) 来促进细胞间的通信.
- 外体miRNAs在食道状细胞癌 (ESCC) 进展中的确切作用仍然不完全理解.
- 这项研究研究了miR-196a-5p在ESCC中的功能.
研究的目的:
- 为了阐明外体miR-196a-5p对ESCC进展的影响.
- 确定miR-196a-5p在ESCC中的作用背后的分子机制.
- 根据miR-196a-5p.p.探索ESCC潜在的治疗点.
主要方法:
- 在ESCC组织,细胞和外体细胞中对miR-196a-5p表达的定量分析.
- 在miR-196a-5p操纵后进行体外功能测定 (细胞生长,迁移,入侵).
- 研究miR-196a-5p的外体介导转移及其对受体细胞的影响.
- 使用分子技术识别miR-196a-5p的直接目标.
- 在体内异种移植瘤模型中,评估针对miR-196a-5p.p的治疗潜力.
主要成果:
- 发现miR-196a-5p在ESCC组织,细胞和外体细胞中被丰富.
- 耗尽miR-196a-5p抑制了ESCC细胞的增殖,迁移和入侵;过度表达促进了这些过程.
- 外体 miR-196a-5p 转移增强了受体 ESCC 细胞中的增殖和迁移.
- 综合膜蛋白2B (ITM2B) 被确定为miR-196a-5p的直接目标.
- 沉默ITM2B部分扭转了miR-196a-5p抑制剂的抑制作用.
- 在体内,降低miR-196a-5p水平导致更小的异种移植瘤.
结论:
- 外体和细胞内miR-196a-5p在促进ESCC生长和迁移方面发挥着至关重要的作用.
- miR-196a-5p/ITM2B轴代表了ESCC病变发生的一个新机制.
- 这些发现表明miR-196a-5p和ITM2B是ESCC治疗的潜在治疗点.
相关概念视频
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
The Tumor Microenvironment
6.6K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.6K
Mitogens and the Cell Cycle
6.4K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.4K
Induced Pluripotent Stem Cells
4.0K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.0K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K

